CXCR3阻断剂Genistein对大鼠胰腺移植后排斥反应的影响

目的 探讨Genistein对大鼠移植胰腺急性排斥反应的影响以及作用机制.方法 以Wistar和SD大鼠为供体,SD大鼠为受体,建立胰腺移植模型.分成同种系移植组、异种系移植组和Genistein治疗组(在异种系移植后每天腹腔注射Genistein 10 mg/kg体重,共7 d),每组lO只.术后7 d切除移植物行病理学观察及CXCR3免疫组化染色,抽血检测血清IFN-γ、IL-2浓度.结果 同种系移植组移植物无急性排斥反应,无CXCR3表达.血清IFN-γ、IL-2浓度分别为(2.76±0.66)pg/ml和(11.83±1.86)pg/ml;异种系移植组的移植胰腺发生强烈的镜下排斥反应,CXCR3强阳性表达,血清IFN-γ、IL-2浓度分别为(32.64±2.52)PS/ml和(29.59±1.71)PS/ml,较同种系移植组显著升高(P<0.05);Genistein治疗组的移植胰腺排斥反应减轻,CXCR3弱阳性表达,血清IFN-γ、IL-2浓度分别为(15.94±1.95)Pg/ml和(22.62±1.76)ps/ml,较异种系移植组显著降低(P<0.05),但高于同种系移植组(P<0.05).结论 Genistein通过阻断CXCR3的表达,可有效减轻胰腺移植后急性排斥反应.

Effects of the CXCR3-inhibitor Genistein on the acute rejection of pancreas transplantation in a rat model

Objective To investigate the effects and mechanisms of the CXCR3-inhibitor genistein on the acute rejection reaction of pancreas transplantation in a rat model. Methods Three groups of rats underwent pancreas transplantation, group one: the syngeneic transplantation group; group two: heterogous transplantation group without genistein-treatment; and group three: genistein-treatment (10 mg/kg,i. p.×7d) transplantation group. The grafts of three groups were harvested at day 7 after operation for histopathology and immunohistochemistry analysis of CXCR3, the serum levels of IFN-γ, IL-2 were examined by ELISA. Results There was no acute rejection reaction of pancreas transplantation in group one and no expression of CXCR3 ,the serum levels of IFN-γ,IL-2 were (2.76±0.66) pg/ml and (11.83±1.86) pg/ml. In group two, there was significant acute rejection reaction and strong expression of CXCR3, the serum levels of IFN-γ, IL-2 were (32.64±2.52) pg/ml and (29.59±1.71 ) pg/ml, which were significantly higher than those in group one (P<0.05). Compared with group two, the damages of graft tissue in genistein-treatment group alleviated and the lymphocytes infiltration decreased, the expression of CXCR3 was weakly positive, the serum levels of IFN-γ, IL-2 were (15.94±1.95) pg/ml and (22.62±1.76) pg/ml, which were significantly lower than those in group two(P<0.05), but was higher than those in group one (P<0.05). Conclusions Genistein could mitigate the acute rejection of pancreas transplantation effectively through inhibiting CXCR3 expression.