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抗氧化剂拮抗紫外线致DNA损伤作用的研究
引用本文:李晶, 刘扬, 王秉贤, 张守平. 抗氧化剂拮抗紫外线致DNA损伤作用的研究[J]. 中国公共卫生, 2002, 18(8): 942-943. DOI: 10.11847/zgggws2002-18-08-24
作者姓名:李晶  刘扬  王秉贤  张守平
作者单位:1.中国医科大学公共卫生学院 沈阳110001;;2.沈阳市疾病预防控制中心
摘    要:目的 探讨抗氧化剂-过氧化氢酶、还原型谷胱甘肽和β-胡萝卜素是否对紫外线照射诱发的DNA损伤具有拮抗作用.方法 提取人外周血中的淋巴细胞,用单细胞凝胶电泳法检测经不同浓度过氧化氢酶、还原型谷胱甘肽或β-胡萝卜素处理及应用不同计量紫外线照射后淋巴细胞的DNA损伤状况.结果 随着紫外线照射剂量的增加,淋巴细胞的DNA损伤程度逐渐加重;外源性过氧化氢酶、还原型谷胱甘肽和β-胡萝卜素的加入可明显减轻DNA损伤程度,但均未能完全阻断DNA的损伤;在一定范围内,这些抗氧化剂的剂量与拮抗功效呈量效关系.结论 过氧化氢酶、还原型谷胱甘肽和β胡萝卜素对紫外线照射诱发的DNA损伤均有拮抗作用,是拮抗紫外线照射诱发DNA损伤较为理想的拮抗剂;单细胞凝胶电泳法适用于研究紫外线照射引起的DNA损伤.

关 键 词:紫外线照射  抗氧化剂  DNA损伤
文章编号:1001-0580(2002)08-0942-02
收稿时间:2002-02-19
修稿时间:2002-02-19

Experimental Study on Effects of Antioxidants on Ultraviolet Radiation-Indued DNA Damage
LI Jing, LIU Yang, WANG Bing-xian, . Experimental Study on Effects of Antioxidants on Ultraviolet Radiation-Indued DNA Damage[J]. Chinese Journal of Public Health, 2002, 18(8): 942-943. DOI: 10.11847/zgggws2002-18-08-24
Authors:LI Jing  LIU Yang  WANG Bing-xian
Affiliation:1.College of Public Health, China Medical University Shenyang 110001, China
Abstract:Objective To investigate the role of antioxidants-catalase,reduced glutathione and β-carotin in UVR-induced DNA damage.Methods After extracting human peripherial blood lymphocytes and treating with catalase,reduced glutathioneβ,-carotin as well as ultraviolet radiation,the damaged degree of lymphyocytic DNA was examined with single cell gel electrophoresis.Results With the UVR dose increasing,the damaged degree of lymphocytic DNA increase gradully;after treating with three antioxidants UVR-induced DNA damage was reduced and its effects showed dose-dependent manner within a certain range.Conclusions Catalase,reduced glutathione andβ-carotin were perfect antagonists to UVR-induced DNA damage;single cell gel electrophoresis was an ideal method of analyzing UVR-induced DNA damage.
Keywords:UVR  antioxidant  DNA damage
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