| 中药抗炎作用与对大鼠腹腔巨噬细胞产生白三烯B_4及5－HETE的影响 |
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| 引用本文： | 翁福海,胡萍,焦建杰. 中药抗炎作用与对大鼠腹腔巨噬细胞产生白三烯B_4及5－HETE的影响[J]. 天津医科大学学报, 1996, 0(1) |
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| 作者姓名： | 翁福海 胡萍 焦建杰 |
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| 摘 要： | 本文建立了提取与测定大鼠腹腔巨噬细胞（ＰＭφ）生物合成白三烯Ｂ_４（ＬＴＢ_４）及５－羟基二十碳四烯酸（５－ＨＥＴＥ）的高效液相色谱法，并研究了４个单味中药抗炎作用与对ＰＭφ产生ＬＴＢ_４及５－ＨＥＴＥ的影响。结果表明大黄、厚朴、白头翁及败酱草提取物对大鼠ＰＭφ合成以上二种介质均有明显抑制作用。对炎性介质ＬＴＢ_４产生的抑制率（％）分别为：大黄素（终浓度取５．０×１０~（－６）ｍｏｌ／Ｌ）为８８．０％；厚朴为９９．８％；白头翁为９４．９％及败酱草为５７．９％（以上终浓度均取５．９×１０~（－２）ｇ／Ｌ）。
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| 关 键 词： | 白三烯B_4；5－羟基二十碳四烯酸；反相高效液相色谱 |
EFFECT OF ANTI-INFLAMMATORY ACTION OF CHINESE HERBS ON THE SYNTHESIS OF LTB4 AND 5-HETE OF PERITONEAL MACROPHAGES IN RATS |
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| Weng Fuhai et al. EFFECT OF ANTI-INFLAMMATORY ACTION OF CHINESE HERBS ON THE SYNTHESIS OF LTB4 AND 5-HETE OF PERITONEAL MACROPHAGES IN RATS[J]. Journal of Tianjin Medical University, 1996, 0(1) |
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| Authors: | Weng Fuhai et al |
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| Affiliation: | Department of Pharmacology |
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| Abstract: | LTB4 and 5-HETE were determined by High Performance Liquid Chromatography,The anti-inflammatory action of four extractions from Chinese herbs including Emodin,Magnolia Officinalis,Pulsatilla Chinensis and patrinia vilosa,was investigated for its effect on the synthesis of LTB4 and 5-HETE released form peritoneal macrophages stimulated by calcium ionophore A23187,The results showed that the four herb extractions significantly inhibited the biosynthesis of both LTB4 and 5-HETE,the inhibite rate was from 60%to 98%as same as that of flufenamic acid.We suggested that the mechanism of antiinflammatory action of these agents may be related to the suppresion of lipoxygenase. |
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| Keywords: | Chinese herb leulotriene B4 5-Hydroxysatetraenoic acid High Performance Liquid Chromatography. |
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