经前期综合征肝气逆、郁两证大鼠模型建立及宏观评价方法初探

目的改进经前期综合征肝气逆、郁两证大鼠模型建立及探讨宏观评价方法，为情志病证发病机理研究提供可资利用的证候动物模型。方法应用行为学判定结合改良阴道涂片法确定大鼠动情周期，采用昼夜颠倒配合旷场法筛选行为学得分相近大鼠进入实验减小误差，调整造模刺激强度及持续时间，应用昼夜颠倒配合旷场法对模型大鼠宏观表现予以量化评价。结果与正常对照纽比较大鼠造模后逆模纽水平得分、垂直得分及旷场实验总分均显著增加（P〈0.05），而郁模组大鼠则显著减少（P〈0.05），用药后这一趋势得到明显纠正（P〈0.05）。结论大鼠模型较好模拟了PMS病证临床表现及病理变化，造模方法成熟可靠，具有较好可信度及可重复性；应用旷场法可明确鉴别肝气逆、郁两证大鼠模型，为证候动物模型中医辨证提供直接依据。

Establishment of Premenstrual Syndrome Liver-qi Invasion, Depression Rats Model and Macro-evaluation

Objective Improve the method of making model and macro-evaluation about Premen- strual Syndrome Liver-qi Invasion, Depression Rats Model, providing a “zheng” animal model for the research of mechanism on emotional disease. Methods Adopted ethology and improved vaginal smear to determine rats oestrous cycle, apply open-field method to flit similar scoring rats entering experiment, adjust stimulus intensity and persistence time, introduce open-field method to estimate rats macroscopic appearance. Results Liver-qi invasion rats＇ scores were much higher than the control while liver-qi depression rats＇ scores decreasing notably, the trend was retrieved after administration. Conclusion The rat model were consistent with clinical manifestation and pathological changes satisfactorily. Modeling method was reliable and mature with a better credibility and repeatability. Adopting open-field method could distinguish syndrome belongings of rats model clearly. Thus provided a direct evidence for differ-entiation of symptoms and signs on animal models.