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| DMSO对EC9706细胞端粒酶活性及生长的影响 | |
| 引用本文： | 刘先军,彭吉霞,吴清明,刘重贞,于皆平.DMSO对EC9706细胞端粒酶活性及生长的影响[J].山西医科大学学报,2003,34(2):106-108. |
| 作者姓名： | 刘先军 彭吉霞 吴清明 刘重贞 于皆平 |
| 作者单位： | 1. 郧阳医学院附属太和医院消化内科,十堰,442000 2. 郧阳医学院机能学实验中心 3. 武汉大学人民医院消化内科 |
| 基金项目： | 郧阳医学院附属太和医院科研经费资助项目 |
| 摘 要： | 目的 研究二甲基亚砜 (DMSO)对EC970 6细胞端粒酶活性及生长的影响。方法 用 1.3 %DMSO处理食管癌EC970 6细胞 ,TRAP -ELISA法检测细胞端粒酶活性的改变 ,流式细胞仪分析细胞周期的改变。结果 DMSO处理后的EC970 6细胞 ,端粒酶活性明显下降 ,且细胞周期明显改变 ,G1期细胞比率显著增高 ,S期细胞数显著降低。结论 DMSO可抑制EC970 6细胞端粒酶活性 ,使EC970 6细胞生长停滞于G1期 ,并诱导细胞凋亡。提示DMSO可用于食管癌的治疗 |
| 关 键 词： | 二甲亚砜 食管肿瘤 细胞周期 细胞分化 端粒 末端转移酶 |
| 文章编号： | 1007-6611(2003)02-0106-03 |
| 修稿时间： | 2003年1月16日 |
Effects of dimethyl sulfoxide on telomerase activity and growth of EC9706 cells |
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| LIU Xian-jun,PENG Ji-xia,WU Qing-ming,et al.Effects of dimethyl sulfoxide on telomerase activity and growth of EC9706 cells[J].Journal of Shanxi Medical University,2003,34(2):106-108. | |
| Authors: | LIU Xian-jun PENG Ji-xia WU Qing-ming |
| Abstract: | Objective To Study the effects of dimethyl sulfoxide on telomerase activity and growth of EC9706 cells. Methods EC9706 cells were treated with 20 ml/L dimethyl sulfoxide (DMSO). Telomerase activity was measured using the telomeric repeat amplification protocol assay and enzyme-linked immunoadsordent assay (TRAP-ELISA), and cell cycle phases were analyzed by flow cytometry(FCM). Results Telomerase activity of treated cells was significantly decreased compared with the control group. FCM data showed that the G1 phase ratio was increased significantly and the S phase ratio was decreased obviously. Conclusion DMSO can inhibit telomerase activity of esophageal carcinoma EC9706 cells and block cell cycle progression at G1 phase, inducing cells apoptosis. These suggest that DMSO can be used in therapy of esophageal carcinoma. |
| Keywords: | dimethyl sulfoxide esophageal neoplasms cell cycle cell differentiation telomerase |
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