| 基于栅藻延迟发光表征中药药性的方法学验证 |
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| 引用本文： | 闵令圆,庞靖祥,杨美娜,周宝宸,荆绮,韩金祥. 基于栅藻延迟发光表征中药药性的方法学验证[J]. 生物医学工程研究, 2020, 39(1): 90-94 |
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| 作者姓名： | 闵令圆 庞靖祥 杨美娜 周宝宸 荆绮 韩金祥 |
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| 作者单位： | 济南大学 山东省医学科学院 医学与生命科学学院,济南250020;山东省医药生物技术研究中心 山东第一医科大学(山东省医学科学院),济南250062;山东省医药生物技术研究中心 山东第一医科大学(山东省医学科学院),济南250062 |
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| 基金项目： | 国家国际科技合作专项资助项目(2014DFA30380);山东省重点研发计划项目(2019GSF108168,2019LYXZ037);山东省医学科学院院级科技计划项目(2018-61)。 |
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| 摘 要： | 对栅藻延迟发光定量化表征中药寒热药性的方法进行优化,并进行方法学验证。用YPMS-2生物光子测量仪检测栅藻激发延迟发光强度,对测得的激发延迟发光强度用Statistica 10.0软件进行线性拟合,得到表征栅藻性质的可靠参数K,用产自于道地产地的经典寒性中药黄芩作为样本,验证本研究方法的精密度、稳定性、重复性,并进行空白实验。栅藻最佳使用K值波动范围是2.81~3.89,本研究方法精密度高,重复性、稳定性良好,空白溶剂无干扰。
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| 关 键 词： | 栅藻 方法学验证 生物光子 生物指示剂 中药药性 延迟发光 |
Methodological verification of characterization of traditional Chinese medicine based on delayed luminescence of scenedesmus |
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| MIN Lingyuan,PANG Jingxiang,YANG Meina,ZHOU Baochen,JING Qi,HAN Jinxiang. Methodological verification of characterization of traditional Chinese medicine based on delayed luminescence of scenedesmus[J]. Journal Of Blomedical Englneerlng Research, 2020, 39(1): 90-94 |
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| Authors: | MIN Lingyuan PANG Jingxiang YANG Meina ZHOU Baochen JING Qi HAN Jinxiang |
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| Affiliation: | (School of Medicine and Life Sciences,University of Jinan-Shandong Academy of Medical Sciences,Jinan 250020,China;Shandong Medicinal Biotechnology Center,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250062) |
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| Abstract: | The method of quantitatively characterizing the cold and hot medicinal properties of Chinese medicinal herbs was optimized for delayed luminescence,and methodological verification was carried out.The YPMS-2 biophoton measuring instrument was used to detect the delayed luminescence intensity of the S.cerevisiae.The measured excitation delay luminescence intensity was linearly fitted with Statistica 10.0 software to obtain a reliable parameter K for characterizing the properties of S.cerevisiae.The classical cold Chinese medicine Astragalus membranaceus was used as a sample to study the precision,stability,and repeatability of the method and to carry out blank experiments.The optimum K-value fluctuation range of Scenedesmus is 2.81~3.89.The method has high precision,good repeatability and stability,and no interference from blank solvent. |
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| Keywords: | Scenedesmus Method validation Biophotonics Biological indicators Traditional Chinese medicine Delayed luminescence |
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