Effects of the Cell Cycle Inhibitor Olomoucine on Inflammatory Response and Neuronal Cell Death after Spinal Cord Injury in Rats |
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| Authors: | TIAN Dai-shi XIE Min-jie YU Zhi-yuan ZHANG Qiang WANG Yi-hui CHEN Bin CHEN Chen WANG Wei |
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| Affiliation: | Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | Objective:The influence of olomoucine on microglial proliferation with associated inflammatory response after spinal cord injury has been determined.Methods:Microglial proliferation and neuronal apoptosis were observed by immunofluorescence.Level of the proinflammatory cytokine interleukin-1β (IL-1β) expression in the injured cord was determined by Western blot analysis.Results:the cell cycle inhibitor olomoucine,administered at 1 h post injury,significantly suppressed microglial proliferation and produced a remarkable reduction of tissue edema formation.In the olomoucine-treated group,a significant reduction of activated and/or proliferated microglial induced IL-1β expression was observed 24 h after SCI.Moreover,olomoucine evidently attenuated the number of apoptotic neurons after SCI.Conclusion:Our findings suggest that modulation of microglial proliferation with associated proinflammatory cytokine expression may be a mechanism of cell cycle inhibition-mediated neuroprotections in the CNS trauma. |
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| Keywords: | cell cycle spinal cord injury microglial cell inflammatory response neuronal apoptosis |
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