| M3R介导逼尿肌细胞收缩与信使分子IP3关系的实验研究 |
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| 引用本文： | 卢根生,宋波,金锡御,熊恩庆.M3R介导逼尿肌细胞收缩与信使分子IP3关系的实验研究[J].中国现代医学杂志,2002,12(24):33-34,37. |
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| 作者姓名： | 卢根生 宋波 金锡御 熊恩庆 |
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| 作者单位： | 第三军医大学西南医院泌尿中心,重庆,400038 |
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| 基金项目： | 国家自然科学基金资助 (3 9770 471) |
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| 摘 要： | 目的 :探讨信使分子IP3 在M3 R介导逼尿肌细胞收缩中的作用。方法 :MR非选择性的激动剂 (carba chol)、拮抗剂 (atropine)及M2 R拮抗剂 (methoctramine)、M3 R拮抗剂 (4-DAMP)刺激原代培养人逼尿肌细胞 ,通过 3 H]掺入法 ,检测磷脂酰肌醇 (PI)的代谢产物 3 H]-IP含量。结果 :3 H]-IP的含量随carbachol刺激浓度的增加而增加 ;atropine和 4 -DAMP能显著抑制carbachol诱导的代谢反应 (P <0 .0 1) ,而methoc tramine不能显著抑制carbachol的作用 ;atropine与 4 -DAMP的作用相比无显著性差异 (P >0 .0 5 )。结论 :M3 R与PI分解产生的第二信使分子IP3 密切相关。
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| 关 键 词： | 逼尿肌细胞 毒蕈碱受体 亚型 信号分子 |
RELATIONSHIP BETWEEN IP3 AND DETRUSOR CELL CONTRACTION MEDIATIED BY M3R |
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| Lu Gensheng,Song Bo,Jin Xiyu,et al..RELATIONSHIP BETWEEN IP3 AND DETRUSOR CELL CONTRACTION MEDIATIED BY M3R[J].China Journal of Modern Medicine,2002,12(24):33-34,37. |
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| Authors: | Lu Gensheng Song Bo Jin Xiyu |
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| Institution: | Lu Gensheng,Song Bo,Jin Xiyu,et al. Department of Urology,Southwestern Hospital,Third Military Medical University,Chongqing 400038$$$$ |
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| Abstract: | Objective:To study the relationship between IP 3 and detrusor cell contraction mediatied by M 3R. Methods:The -IP contents of human cultured detrusor cells were detected after stimulated by carbachol, atropine, methoctramine, 4-DAMP respectively.Results:The -IP contents increased with carbachol concentration. Both atropine and 4-DAMP signficantly inhibited the effect of carbachol on PI decomposition ( P < 0.01), but methoctramine didn't; The effect on PI decomposition between atropine and 4-DAMP had no significant difference ( P >0.05).Conclusion: M 3R is much related to IP 3. |
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| Keywords: | Detrusor Cell Muscarinic Receptor Subtype Signal Moleculer |
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