| 载10-羟基喜树碱靶向脂质微泡的制备及体外寻靶能力实验研究 |
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| 引用本文： | 骆杰,王志刚,张群霞,钟世根,张辉.载10-羟基喜树碱靶向脂质微泡的制备及体外寻靶能力实验研究[J].中国医学影像技术,2010,26(3):418-420. |
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| 作者姓名： | 骆杰 王志刚 张群霞 钟世根 张辉 |
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| 作者单位： | 1. 重庆医科大学超声影像学研究所,重庆,400010
2. 重庆医科大学附属第二医院超声科,重庆,400010 |
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| 基金项目： | 国家自然科学基金面上项目 |
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| 摘 要： | 目的 制备携人肝癌单抗Hab18载10-羟基喜树碱的脂质微泡,并观察其体外寻靶能力.方法 制备生物素化抗人肝癌Hab18单克隆抗体,检测其生物素化程度;用机械振荡法制备载药脂质微泡,以生物素-亲和素桥连方式构建携人肝癌单抗Hab18载10-羟基喜树碱的脂质超声微泡,检测其一般特性、包封率和载药量,以免疫荧光法检测微泡与抗体的连接情况,在光镜下观察靶向载药微泡的寻靶能力,并与载药非靶向微泡进行比较.结果 每个单抗分子平均可与13个生物素分子结合.载药靶向脂质超声微泡分布均匀,平均粒径为1.52 μm,包封率为76.32%,载药量为21.81%.免疫荧光法显示微泡表面可见明亮的红色环状荧光,体外寻靶实验显示该载药靶向微泡可与人肝癌7721细胞牢固结合.结论 携人肝癌单抗Hab18载10-羟基喜树碱的脂质超声载药靶向微泡可成功制备,其包封率和载药量较高,具有较强的体外寻靶能力.
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| 关 键 词： | 10-羟基喜树碱 抗体 单克隆 微泡 |
| 收稿时间： | 2009/9/28 0:00:00 |
| 修稿时间： | 2009/11/18 0:00:00 |
Experimental study on preparation and targeting ability in vitro of HCC-targeted lipid microbubble containing 10-HCPT |
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| LUO Jie,WANG Zhi-gang,ZHANG Qun-xi,ZHONG Shi-gen and ZHANG Hui.Experimental study on preparation and targeting ability in vitro of HCC-targeted lipid microbubble containing 10-HCPT[J].Chinese Journal of Medical Imaging Technology,2010,26(3):418-420. |
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| Authors: | LUO Jie WANG Zhi-gang ZHANG Qun-xi ZHONG Shi-gen and ZHANG Hui |
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| Institution: | Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China;Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China;Department of Ultrasonography, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China;Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China;Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China |
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| Abstract: | Objective To prepare HCC-targeted lipid ultrasound microbubble containing 10-hydroxycamptothecin(10-HCPT),and to assess its targeting function in vitro.Methods After the biotinylated monoclonal antibody Hab18 was prepared,the biotinylated degree was determined.Microbubbles containing 10-HCPT were prepared by mechanical vibration.Then the biotinylated antibody was attached to the surface of the microbubbles by avidin-biotin interaction.The physical property,entrapment efficiency and the drug-loading amounts ... |
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| Keywords: | 10-hydroxycamptothecin Antibodies monoclonal Microbubbles |
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