Common studied polymorphisms do not affect plasma cytokine levels upon endotoxin exposure in humans |
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Authors: | Taudorf S Krabbe K S Berg R M G Møller K Pedersen B K Bruunsgaard H |
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Institution: | Department of Infectious Diseases, Centre of Inflammation and Metabolism, Rigshiospitalet, Unviersity of Copenhagen, Faculty of Health Sciences, Copenhagen, Denmark. sarahtaudorf@dadlnet.dk |
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Abstract: | The aim of this study was to investigate to what extent single nucleotide polymorphisms (SNPs) in promoter regions of genes of Toll-like receptor (TLR)-4, tumour necrosis factor (TNF)-alpha, interleukin (IL)-18, interferon (IFN)-gamma, IL-6 and IL-10 affect the cytokine response during a controlled low-grade inflammatory response in vivo. Two hundred healthy young male volunteers were genotyped, and cytokine levels were measured in response to a low-dose intravenous bolus of Escherichia coli endotoxin. No association was detected between SNPs (TLR-4299, TLR-4399, TNF-308, IL-18-137, IL-18-607, IFN-gamma+874, IL-6-174, IL-10-592 and IL-10-1082) and endotoxin-induced changes in plasma levels of TNF-alpha, IL-6 and IL-10. IL-18 levels were unaffected by endotoxin. In conclusion, the investigated SNPs did not affect endotoxin-induced low-grade cytokine production of TNF-alpha, IL-6, IL-18 or IL-10 in healthy young men. Previous reports of a major heritability factor in the inflammatory response may be due to other target genes or effects in older age groups or women. |
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Keywords: | cytokine human endotoxaemia low-grade inflammation single nucleotide polymorphism |
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