雌激素对大鼠局灶性脑缺血神经细胞caspase-3表达的影响

目的:探讨雌激素对局灶性脑缺血大鼠神经细胞caspase-3表达的影响。方法:健康雌性Wistar大鼠30只,随机等分为假手术组、缺血对照组及雌激素用药组。采用栓线法阻断大脑中动脉建立局灶性脑缺血模型。缺血2 h再灌注24 h后断头取脑,用体积分数为1%四氮唑染色分别观察各组大鼠脑梗死灶的大小,应用免疫组织化学方法检测大鼠脑组织caspase-3的表达。结果:假手术组未发现脑梗死灶和caspase-3表达阴性;雌激素用药组(16.7±2.1)%脑梗死体积明显小于缺血对照组(21.9±4.3)%(P<0.01);caspase-3的表达雌激素用药组(131±15)个明显小于缺血对照组(86±11)个(P<0.01)。结论:雌激素可抑制大鼠脑神经细胞caspase-3活性,减少脑神经元凋亡,具有脑神经保护作用。

Effect of estrogen on the expression of caspase-3 in nerve cell after focal cerebral ischemia-reperfusion in rats

Objective: To investigate the effect of estrogen on the expression of caspase-3 in rat focal cerebral ischemia tissue.Methods: A total of 30 health female Wistar rats were randomly divided into sham operation group,ischemic control group,estrogen group.Focal cerebral ischemia model was established with the middle cerebral artery occlusion for 2 hours by reversible inserting a nylon thread method.All animals were killed at 24 hours of reperfusion.The infarct volume of each group were measured by 1% 2,3,5-triphenyltetrazolium chloride(TTC) staining technique and the expression of caspase-3 was measured by using immunohistochemistry staining.Results: The sham operation group could not find infarction focus and caspase-3 expression.There was remarked difference on the infarct volume between the estrogen groups(16.7± 2.1)% and the ischemic control group(21.9 ± 4.3)%(P<0.01).The expression of caspase-3 reduced significantly in estrogen group(86±11) compared with ischemic control group(131±15)(P<0.01).Conclusion: The expression of caspase-3 remarkablely increases after reperfusion,and estrogen therapy could inhibit the expression of caspase-3 and reduced the neuronal cell apoptosis in rats.Estrogen provides neuroprotective in rats.