FRNK质粒转染抑制肝星状细胞胶原合成

目的：探讨黏着斑激酶相关非激酶（FRNK）对纤维连接蛋白（FN）刺激的肝星状细胞（HSCs）胶原合成的影响。方法:应用体外细胞培养技术，脂质体介导法进行FRNK质粒瞬时转染；采用Western blotting方法测定FRNK蛋白在HSCs的表达，鉴定转染效果；［3H］-Pro掺入法检测HSCs总胶原和Ⅰ型胶原的合成能力。结果:FRNK质粒成功转染HSCs，于转染48 h时FRNK蛋白表达最强（P<0.05）；在FRNK转染HSCs 48 h后总胶原合成能力（498.17±73.20）较空质粒组（748.33±61.30）显著下降（P<0.01）；在FRNK转染HSCs 48 h后 Ⅰ型 胶原合成能力（163.17±23.80）较空质粒组（371.58±15.44）亦显著下降（P<0.01）。结论:FRNK可以使HSCs总胶原和Ⅰ型胶原合成能力降低，提示其对肝星状细胞胶原合成功能具有负调控作用。

Inhibitory effects of FRNK on collagen synthesis in hepatic stellate cells

AIM:To investigate the effect of focal adhesion kinase-related non-kinase (FRNK) on collagen synthesis in hepatic stellate cells (HSCs).METHODS:HSCs were transfected with FRNK by cationic liposome method. The expressions of FRNK at the protein level in HSCs were detected by Western blotting analysis. HSCs collagen synthesis capability was examined by［3H］-Pro incorporation assay.RESULTS:The exposure of HSCs to FRNK led to the up-regulated expression of FRNK protein, and it was at 48 h after transfection that the FRNK protein content was the highest. Moreover, after FRNK was transfected successfully in HSCs, the total collagen synthesis and type I collagen synthesis were inhibited.CONCLUSION:After FRNK is transfected successfully in HSCs using Lipofectamine, the synthesis of total and type I collagen in HSCs is inhibited.