前列腺癌组织中核干因子基因表达的临床意义

目的探讨核干因子（nucleostemin gene，NS）基因在前列腺癌（prostate cancer，PCa）组织中的表达及其与血清前列腺特异性抗原（prostate specific antigen，PSA）的关系。方法采用逆转录-聚合酶链反应（RT—PCR）及免疫组织化学法检测NS在PCa及良性前列腺增生（BPH）组织中的表达，分析其在PCa中的表达水平与血清PSA的关系。结果45例PCa组织标本中，NSmRNA的阳性表达率为80．0％（36／45）。20例BPH组织中均未见阳性表达。在高分化、中分化、低分化PCa组织中NS蛋白阳性表达例数分别为4、19、13例。NS蛋白表达与PCa恶性程度呈正相关（r=0．954，P〈0．05），与患者血清PSA水平无明显相关性（r=0．237，P〉0．05）。结论NS蛋白表达与PCa的分级密切相关，可作为一种诊断PCa的新肿瘤标志物。

Expression of nucleostemin gene in prostate cancer and its correlation with prostate specific antigen

Objective To investigate the expression of nucleostemin gene(NS)in prostate cancer and its correlation with prostate specific antigen(PSA).Methods The expressions of nucleostemin mRNA and protein were measured by RT-PCR and immunohistochemical stain in 20 specimens of benign prostate hyperplasia and 45 specimens of prostate cancer,and the relation between their levels and serum PSA was analyzed.Results NS gene expression was detected in 36 specimens of prostate cancer(80.0%).The positive expression of NS gene was in 4,19,13 specimens of high,moderate,low grade of prostate cancer.The expression of nucleostemin gene was related significantly to the tumor grade(r=0.954,P<0.05),and no significant relationship between nucleostemin expression and PSA was found(r=0.237,P>0.05).Expression of nucleostemin gene was not detected in benign prostate hyperplasia tissues.Conclusion The expression of nucleostemin gene in prostate cancer is closely correlated with the grading of tumors and may serve as a marker in estimating the malignancy of prostate cancer.