软组织平滑肌肉瘤中肿瘤抑制基因p15,p16的表达

目的：探讨p15,p16蛋白在软组织平滑肌肉瘤（LMS）及平滑肌瘤（LM）的表达情况及其意义，方法：采用免疫组织化学方法检测38例LMS及20例LM标本的p15,p16蛋白表达情况，结果：在LMS，p15,p16的阴性率分别为18．42％及42．1％，二者差异有显著性。p15在LM的阴性率为60％，高于LMS。38例LMSp15和p16总异常率为52．6％，其中Ⅰ级LMS异常率显著高于Ⅱ、Ⅲ级。随访15例LMS，复发死亡组p15和p16异常率达75％，高于无复发组。结论：在LMSp16表达缺失比p15缺失更为常见，p15的缺失在LM在比在LMS常见，LMS的发生发展及预后p15及p16共同失活有关。

Expression of tumor suppressor gene p15, p16 in leiomyosarcoma of soft tissue

Purpose　To investigate the significance of tumor suppressor gene p15 and p16 expression in soft tissue leiomyosarcoma(LMS) and leiomyoma(LM). Methods　Expressions of p15 and p16 was studied in 38 cases of LMS and 20 LM with immunohistochemical method. Results　The abnormal expression rates of p15 and p16 in LMS were 18.4% and 42.1% respectively, and were significantly different. p15 abnormal expression rate in LM was 60%, significantly higher than in LMS. The total abnormal expression rate of p15 and p16 was 52.6% in LMS studied. Grade Ⅰ tumor had the significantly higher abnormal expression rate than that of grade Ⅱ or Ⅲ tumor. Fifteen cases of LMS were followed. The abnormal expression rate of p15 and p16 in recurrent group was 75%, significantly higher than that in non recurrent group. Conclusions　Loss of expression of p16 protein is more frequent in LMS than that of p15, whereas deletion of p15 protein is more frequent in LM than in LMS. Loss of functions of p15 together with p16 participates in the tumorigenesis and progression of LMS, and is correlated with pathologic grading and prognosis of LMS.