Isolation and characterization of subgenomic DNAs encapsidated in "single" T = 1 isometric particles of Maize streak virus |
| |
| Authors: | Casado Carolina G Javier Ortiz G Padron Eric Bean Samantha J McKenna Robert Agbandje-McKenna Mavis Boulton Margaret I |
| |
| Institution: | Department of Biochemistry and Molecular Biology, College of Medicine, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0245, USA. |
| |
| Abstract: | "Single" T = 1 isometric particles of Maize streak virus (MSV) have been isolated from infected maize leaves. Biochemical and genetic characterizations show that these particles contain subgenomic (sg) MSV DNA encapsidated by the MSV coat protein. The largest sg DNA is 1.56 kb, slightly larger than half genome size, although sg DNAs as small as 0.2 kb were also cloned. The sg DNAs are not infectious, and they do not appear to play a role in the pathogenicity of MSV. This is the first report of sg DNAs for MSV and, to our knowledge, the first time that encapsidated sg DNAs have been characterized at the sequence level for any geminivirus. These data will assist in our investigations into the role of genomic DNA in the formation of the unique geminate capsid architecture of the Geminiviridae. |
| |
| Keywords: | Maize streak virus Subgenomic DNAs Isometric particles Geminivirus Coat protein |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
