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| 中国人前列腺特异膜抗原基因真核表达载体的构建和序列测定 | |
| 引用本文： | 曹开源,戴淑琴,徐霖,袁广卿,黄小荣,丘少鹏,郭琳洁.中国人前列腺特异膜抗原基因真核表达载体的构建和序列测定[J].中国病理生理杂志,2004,20(6):1009-1012. |
| 作者姓名： | 曹开源 戴淑琴 徐霖 袁广卿 黄小荣 丘少鹏 郭琳洁 |
| 作者单位： | 1. 中山大学中山医学院免疫教研室, 广东 广州 510089; 2. 中山大学附属第一医院, 广东 广州 510089; 3. 中山大学中山眼科中心, 广东 广州 510089 |
| 基金项目： | 广东省自然科学基金资助项目 (No .0 2 190 7) |
| 摘 要： | 目的:构建中国人前列腺特异膜抗原(PSMA)基因编码区序列的真核表达载体并进行序列测定。方法:从前列腺癌组织中提取总RNA,采用RT-PCR技术扩增前列腺特异膜抗原的基因编码区序列,将序列定向克隆至真核表达载体pcDNA3.0,并进行序列测定。结果:本实验所扩增的中国人PSMA基因编码区序列与Israeli等报道的序列同源性为99.7％,目的基因与载体正确连接。结论:成功克隆了PSMA编码区序列,并构建了其真核表达载体,为PSMA基因修饰的树突状细胞疫苗的前列腺癌生物治疗奠定基础。 |
| 关 键 词： | 前列腺特异抗原 逆转录聚合酶链式反应 克隆 分子 序列分析 |
| 文章编号： | 1000-4718(2004)06-1009-04 |
| 收稿时间： | 2003-5-21 |
| 修稿时间： | 2004-1-8 |
Construction and sequence analysis of eukaryotic expression vector of Chinese prostate-specific membrane antigen |
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| CAO Kai-yuan,DAI Shu-qin,XU Lin,YUAN Guang-qing,HUANG Xiao-rong,QIU Shao-peng,GUO Lin-jie.Construction and sequence analysis of eukaryotic expression vector of Chinese prostate-specific membrane antigen[J].Chinese Journal of Pathophysiology,2004,20(6):1009-1012. | |
| Authors: | CAO Kai-yuan DAI Shu-qin XU Lin YUAN Guang-qing HUANG Xiao-rong QIU Shao-peng GUO Lin-jie |
| Institution: | 1. Department of Immunology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510089, China; 2. The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510089, China; 3. Zhongshan Ophthamic Center, Sun Yat-sen University, Guangzhou 510089, China |
| Abstract: | AIM: To obtain eukaryotic expression vector of Chinese prostate-specific membrane antigen. METHODS: Chinese prostate-specific membrane antigen (PSMA) cDNA was amplified by RT-PCR from prostate cancer tissues, then cloned into eukaryotic expression vector pcDNA3 0 and sequenced. RESULTS: Seven bases in Chinese PSMA cDNA sequence were found different from those reported by Israeli, which lead to two different amino acids. CONCLUSION: We have obtained the PSMA cDNA, and the recombinant eukaryotic expression vector was successfully constructed. The study lays foundation for DCs vaccine modified by PSMA gene for the treatment of prostate neoplasms. |
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