| 天花粉多糖对人外周血单个核细胞的免疫活性作用 |
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| 引用本文： | 徐水凌,赵桂珠,屠婕红,顾少峰,顾超,朱逢佳. 天花粉多糖对人外周血单个核细胞的免疫活性作用[J]. 中国中药杂志, 2010, 35(6): 745-749 |
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| 作者姓名： | 徐水凌 赵桂珠 屠婕红 顾少峰 顾超 朱逢佳 |
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| 作者单位： | 嘉兴学院医学院免疫学教研室,浙江嘉兴,314001 |
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| 基金项目： | 浙江省中医药科技计划项目(2008CA108);嘉兴学院校SRT项目 |
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| 摘 要： | 目的:建立天花粉多糖促人外周血单个核细胞(peripheral blood mononuclear cells,PBMC)淋巴细胞增殖的方法,了解天花粉多糖对淋巴细胞增殖、活化作用,对T淋巴细胞亚群分群的影响和诱导产生TNF-α,IL-6水平的差异。方法:制备天花粉多糖提纯液。健康人PBMC作为药物筛选的靶细胞;采用MTT法测定不同浓度天花粉多糖(0.5,1.0,5.0,10.0,20.0,50.0 mmol.L-1)的促人PBMC淋巴细胞增殖作用;采用流式细胞术和ELISA法分别检测药物作用后人PBMC中T淋巴细胞亚群(CD3+,CD4+,CD8+T细胞)的表达和诱导人PBMC分泌产生TNF-α,IL-6水平。结果:1.0~50.0 mmol.L-1天花粉多糖对人PBMC具有明显促增殖作用(P0.05);经5.0,10.0 mmol.L-1天花粉多糖刺激后的人PBMC中,CD3+,CD4+,CD8+T细胞的含量明显高于对照组(P0.05),1.0,5.0,10.0 mmol.L-1天花粉多糖刺激人PBMC 8 h后,TNF-α,IL-6水平显著升高(P0.05)。结论:天花粉多糖对人PBMC有明显促增殖和活化作用,不同程度地上调T淋巴细胞亚群中CD3+,CD4+,CD8+T细胞的含量,并可诱导人PBMC高水平分泌产生TNF-α,IL-6,为阐明天花粉多糖的免疫活性和作用机制奠定基础。
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| 关 键 词： | 天花粉多糖 淋巴细胞增殖 T淋巴细胞亚群 细胞因子 |
| 收稿时间： | 2009-08-01 |
Immunocompetence effects of polysaccharide of snakegourd root on human peripheral blood mononuclear cells in vitro |
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| XU Shuiling,ZHAO Guizhu,TU Jiehong,GU Shaofeng,GU Chao and ZHU Fengjia. Immunocompetence effects of polysaccharide of snakegourd root on human peripheral blood mononuclear cells in vitro[J]. China Journal of Chinese Materia Medica, 2010, 35(6): 745-749 |
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| Authors: | XU Shuiling ZHAO Guizhu TU Jiehong GU Shaofeng GU Chao ZHU Fengjia |
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| Affiliation: | Department of Immunology, Medical School of Jiaxing College, Jiaxing 314001, China;Department of Immunology, Medical School of Jiaxing College, Jiaxing 314001, China;Department of Immunology, Medical School of Jiaxing College, Jiaxing 314001, China;Department of Immunology, Medical School of Jiaxing College, Jiaxing 314001, China;Department of Immunology, Medical School of Jiaxing College, Jiaxing 314001, China;Department of Immunology, Medical School of Jiaxing College, Jiaxing 314001, China |
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| Abstract: | |
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| Keywords: | polysaccharide of snakegourd root lymphocyte proliferation T lymphocyte subsets cytokines |
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