| 恶性疟原虫红内期145／102kDa抗原的超微结构定位 |
| |
| 引用本文： | 吴莉菊 刘尔翔. 恶性疟原虫红内期145／102kDa抗原的超微结构定位[J]. 中国寄生虫学与寄生虫病杂志, 1993, 11(2): 102-104 |
| |
| 作者姓名： | 吴莉菊 刘尔翔 |
| |
| 作者单位： | 中国预防医学科学院寄生虫病研究所，中国医学科学院基础医学研究所，第二军医大学寄生虫学教研室，中国医学科学院基础医学研究所，中国预防医学科学院寄生虫病研究所 上海 200025，北京 100005，上海 200433，北京 100005，上海 200025 |
| |
| 基金项目： | 联合国开发计划署/世界银行/世界卫生组织热带病研究培训特别规划资助课题(880153) |
| |
| 摘 要： | 用LR White低温包埋法及胶体金标记免疫电镜细胞化学技术对保护性单克隆抗体M26-32识别的体外培养的恶性疟原虫FCC1/HN株红内期145/102 kDa抗原进行超微结构定位研究。发现金颗粒主要定位在该株原虫红内期环状体、滋养体、裂殖体及裂殖子的细胞质中;一些金颗粒则定位于原虫表面的复合膜或散布在感染红细胞的细胞质中。表明145/102kDa抗原是恶性疟原虫FCC1/HN株红内期各无性发育阶段的共同细胞质抗原,其一部分可途经原虫表面的复合膜而被转运到感染红细胞的细胞质。
|
| 关 键 词： | 疟原虫 抗原 胶体金标记 超微结构 |
ULTRASTRUCTURAL LOCALIZATION OF 145/102 kDa ANTIGENS IN ERYTHROCYTIC STAGES OF PLASMODIVM FALCIPARUM |
| |
| WuLi-Ju,Liu Er-Xiang,Miao Wei-Min,Li Wen-Lu,Zhu Zi-Yang Institute of Parasitic Diseases,Chinese Academy of Preventive Medicine,Shanghai. ULTRASTRUCTURAL LOCALIZATION OF 145/102 kDa ANTIGENS IN ERYTHROCYTIC STAGES OF PLASMODIVM FALCIPARUM[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1993, 11(2): 102-104 |
| |
| Authors: | WuLi-Ju Liu Er-Xiang Miao Wei-Min Li Wen-Lu Zhu Zi-Yang Institute of Parasitic Diseases Chinese Academy of Preventive Medicine Shanghai |
| |
| Affiliation: | WuLi-Ju,Liu Er-Xiang,Miao Wei-Min,Li Wen-Lu,Zhu Zi-Yang Institute of Parasitic Diseases,Chinese Academy of Preventive Medicine,Shanghai 200025, Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences,Beijing 100005, Department of Parasitology,Second Military Medical University,Shanghai 200433 |
| |
| Abstract: | The ultrastructural localization of the 145/102 kDa antigens recognized by the possible protective monoclonal antibody (MeAb) M26-32 in erythrocytic stages of Plasmodium falci-parum, FCC1/HN, in vitro, was investigated by immuno-electron microscopy with LR White resin embedding and colloidal gold probe cytochemistry techniques. The results showed that the gold particles were mainly localized within the cytoplasm of ring forms, trophozoites,schizonts and merozoites of the Plasmodium. Some gold particles were found to locate on the pellicular complex of the plasmodium surface or in the cytoplasm of the infected erythrocytes. The results indicated that 145/102 kDa antigens were the common cytoplasmic antigens of asexual blood stages of Plasmodium falciparum, FCC1/HN, while a portion of the antigens could be transported to the cytoplasm of the infected erythrocytes via the pellicular complex of the plasmodium surface (Figs. 1-4). |
| |
| Keywords: | Plasmodium falciparum antigen monoclonal antibody colloidal gold labeling ultrastructural localization |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
