| 人脑胶质瘤细胞系X线敏感性的实验研究 |
| |
| 引用本文： | 沈红,关希丽,关庆柏.人脑胶质瘤细胞系X线敏感性的实验研究[J].哈尔滨医科大学学报,2000,34(2):95-97. |
| |
| 作者姓名： | 沈红 关希丽 关庆柏 |
| |
| 作者单位： | 哈尔滨医科大学附属第一医院X-刀中心!黑龙江哈尔滨150001，哈尔滨医科大学附属第一医院X-刀中心!黑龙江哈尔滨150001，哈尔滨医科大学附属第一医院X-刀中心!黑龙江哈尔滨150001，哈尔滨医科大学附属第一医院X-刀中心!黑龙江哈尔滨150001，哈尔滨医科大学附属第一医院X-刀中心!黑龙 |
| |
| 摘 要： | 目的 研究人脑胶质瘤细胞系对不同强度Ｘ线的敏感性。方法 以ＢＴ３２５细胞系为实验对象，设置了阴性对照、５Ｇｙ、１０Ｇｙ、１５Ｇｙ、２０Ｇｙ、３０Ｇｙ组，应用Ｖａｒｔａｎ直线加速器行单次大剂量照射。采用免疫组法观察Ｐ５３、ＢｃＬ－２和ＰＣＮＡ基因的表达的情况，采用光镜和电镜观察细胞凋亡的形态学改变。结果 ２０Ｇｙ组４８小时时，细胞凋亡发生率最高，此时Ｐ５３基因表达最高、Ｂｃｌ－２基因表达明显减弱，但
|
| 关 键 词： | 脑胶质瘤 细胞凋亡 X线剂量 敏感性 放射疗法 |
Experimental study on sensibility to X-ray of human glioma cell line |
| |
| SHEN Hong,GUAN Xi-li,GUAN
Qing-bai,et al.Experimental study on sensibility to X-ray of human glioma cell line[J].Journal of Harbin Medical University,2000,34(2):95-97. |
| |
| Authors: | SHEN Hong GUAN Xi-li GUAN
Qing-bai |
| |
| Institution: | SHEN Hong,GUAN Xi-li,GUAN
Qing-bai,et al
(Department of X-Knife,The First Affiliated Hospital,Harbin Medical University,Harbin
150001,China) |
| |
| Abstract: | Objective To study human glioma cell line's sensibility to X ray.Methods BT325 cells were divided into 6 groups:5Gy,10Gy,15Gy,20Gy,30Gy and negative control.Varian Clinac600C was used toradiate BT325 cells.Immunohistochemistry(ABC) method was used to examine the expression of p53,BcL 2 PCNA genes.Results We found that 48h after 20Gy radiation,p53 expression reached its peak while BcL 2 expression significantly decreased.PCNA expression was higher at 24h than that at 48h.Conclusion Gene expression of p53,BcL 2 and PCNA are related to the radiobiological effect. |
| |
| Keywords: | glioma cell line apoptosis X ray gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
