上呼吸道脱落细胞支原体抗原检测的临床意义

目的探讨呼吸系肺炎支原体感染的早期诊断。方法采用免疫荧光法（ＩＦＡ），检测呼吸系感染患者的上呼吸道脱落细胞肺炎支原体抗原和血清ＩｇＭ抗体。结果上呼吸道脱落细胞肺炎支原体抗原和血清ＩｇＭ抗体的阳性率分别为１７％和２０％，这些患者符合肺炎支原体感染的临床特点。结论上呼吸道脱落细胞肺炎支原体抗原的检测简单易行，可用于呼吸系肺炎支原体感染的早期诊断，同时检测血清ＩｇＭ抗体可进一步提高诊断的阳性率

The clinical diagnostic significance on detection of mycoplasma pneumoniae antigen in upper respiratory tract epithelium

Objective To investigate the early diagnosis of pulmonary mycoplasma pneumoniae (MP) infection by detection of MP antigen in upper respiratory tract epithelium. Method A prospective study on respiratory MP infection was conducted in 110 patients with acute respiratory infection from February 1996 to November 1997 in our hospital. Upper respiratory tract epithelia were collected from gargle and MP antigen was detected by immunofluorescent assay (IFA).At the same time, serum samples were taken and immunoglobulin M (IgM)fractions of specific antibody to MP were assessed by the same method in 70 of 110 patients. Moreover, clinical features, cold agglutination test and chest X ray were also collected for analysis. Result The positive rates of MP antigens and IgM antibody were 17% and 20%, respectively, while the titer on cold agglutination test >1:8 in only two patients. The clinical features in patients with positive MP antigen and/or IgM antibody were similar to that of pulmonary MP infection. Conclusion Examination of MP antigen in upper respiratory tract epithelium collected from gargle combined with serum specific IgM antibody is a good method for the early diagnosis of pulmonary MP infection.