女性生殖道不同部位检测支原体的序贯试验分析

目的探讨解脲脲原体和人型支原体在女性生殖道的主要寄生部位.方法采用载量一致的标准无菌棉拭子分别于女性阴道穹窿和宫颈口采样,用法国生物梅里埃ID试剂进行解脲脲原体和人型支原体的培养鉴定及半定量计数,按照双向质反应开放型序贯试验设计,对检测结果进行比较分析.结果阴道穹窿部位标本中两种支原体的总阳性数和≥10^4ccu/ml半定量阳性数均高于宫颈口标本.结论对女性进行生殖道支原体感染的检测时应采集阴道穹窿标本,以提高检出率.

Sequential analysis of detection Mycoplasma in different positions of female genital tract

OBJECTIVE: To discuss the main parasitic position of Ureaplasma urealyticum (Uu) and Mycoplasma hominis (Mh) in female genital tract. METHODS: Using the standard aseptic cotton swab to collect secretion in vaginal fornix and orificium internum uteri, to culture Uu and Mh in Mycoplasma ID medium of France Bio-Merieux Co. According to double-direction quality reaction sequential test design, detection results of different position were analyzed. RESULTS: Total positive and > or = 10(4) ccu/ml positive of Uu in vaginal fornix were significantly higher than that in orificium internum uteri. Total positive of Mh in vaginal fornix was significantly higher than that in orificium internum uteri as well. CONCLUSION: In order to raise the detectable rate of Mycoplasma, we suggested that the secretion in vaginal fornix position be collected.