Genetic diversity of folate profiles in seeds of common bean,lentil, chickpea and pea |
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| Affiliation: | 1. Crop Development Centre/Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon SK S7N 5A8, Canada;2. ACRD, National Research Council, 110 Gymnasium Place, Saskatoon SK S7N 4K9, Canada;1. Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7N 0X2, Canada;2. National Research Council of Canada, Saskatoon, SK S7N 0W9, Canada;1. Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, Canada S7N 0X2;2. National Research Council Canada, Saskatoon, Saskatchewan, Canada S7N 0W9;1. Department of Food Science and Technology, Faculty of Nutrition Science, Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Department of Chemistry, Faculty of Science, Alzahra University, Tehran, Iran;3. Department of Microbial Biotechnology and Biosafety, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran |
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| Abstract: | Folates are water-soluble B vitamins and act as cofactors in many metabolic functions in the human body. Pulses have traditionally been considered as a good dietary source of folates. The objectives of this study were (1) to determine the concentration of folates in four cultivars each of common bean, lentil, chickpea and pea, and (2) to determine the effect of growing location on folate concentration. Six folate monoglutamates were quantified by ultra-performance liquid chromatography coupled with mass spectrometry (UPLC–MS/MS). Total folate concentration ranged from 351 to 589 μg/100 g in chickpea, 165 to 232 μg/100 g in common bean, 136 to 182 μg/100 g in lentil, and 23 to 30 μg/100 g in pea. The 5-methyltetrahydrofolate (5-MTHF) and 5-formyltetrahydrofolate (5-FTHF) folates were most abundant in common bean, lentil and chickpea, whereas 5-MTHF and tetrahydrofolate (THF) were the predominant forms in pea. Significant differences were detected among cultivars for all folates across the pulses, except for 5,10-methenyltetrahydrofolate (5,10-MTHF) in lentil, 5-MTHF in chickpea, and 5,10-MTHF and folic acid (FA) in pea. Significant effects for location and cultivar by location were also observed for the majority of the folates. |
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| Keywords: | Cultivars Folates Pulses Liquid chromatography Mass spectrometry Quantification Food analysis Food composition FA" },{" #name" :" keyword" ," $" :{" id" :" kw0050" }," $$" :[{" #name" :" text" ," _" :" folic acid 10-FFA" },{" #name" :" keyword" ," $" :{" id" :" kw0060" }," $$" :[{" #name" :" text" ," _" :" 10-formylfolic acid 5-FTHF" },{" #name" :" keyword" ," $" :{" id" :" kw0070" }," $$" :[{" #name" :" text" ," _" :" 5-formyltetrahydrofolate 5,10-MTHF" },{" #name" :" keyword" ," $" :{" id" :" kw0080" }," $$" :[{" #name" :" text" ," _" :" 5,10-methenyltetrahydrofolate 5-MTHF" },{" #name" :" keyword" ," $" :{" id" :" kw0090" }," $$" :[{" #name" :" text" ," _" :" 5-methyltetrahydrofolate THF" },{" #name" :" keyword" ," $" :{" id" :" kw0100" }," $$" :[{" #name" :" text" ," _" :" tetrahydrofolate UPLC–MS" },{" #name" :" keyword" ," $" :{" id" :" kw0110" }," $$" :[{" #name" :" text" ," _" :" ultra-performance liquid chromatography–mass spectrometry |
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