Entamoeba invadens: Identification of a SERCA protein and effect of SERCA inhibitors on encystation |
| |
| Institution: | 1. Department of Forest Sciences, University of Helsinki, Helsinki, Finland;2. Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland;3. Department of Agricultural Biotechnology, Centers for Fungal Pathogenesis and Fungal Genetic Resources, Seoul National University, Seoul, South Korea;1. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA;1. Department of Immunology, Binzhou Medical University, Yantai, Shandong, 264003, PR China;2. Department of Radiology, Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong, 256603, PR China;3. Department of Gynecology and Obstetrics, Binzhou Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong, 256603, PR China;1. Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, United Kingdom;2. Institute for Animal Health, Compton, Newbury, Berkshire, United Kingdom;1. College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People''s Republic of China;2. Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People''s Republic of China |
| |
| Abstract: | Calcium has an important role on signaling of different cellular processes, including growth and differentiation. Signaling by calcium also has an essential function in pathogenesis and differentiation of the protozoan parasites Entamoeba histolytica and Entamoeba invadens. However, the proteins of these parasites that regulate the cytoplasmic concentration of this ion are poorly studied. In eukaryotic cells, the calcium-ATPase of the SERCA type plays an important role in calcium homeostasis by catalyzing the active efflux of calcium from cytoplasm to endoplasmic reticulum. Here, we reported the identification of SERCA of E. invadens (EiSERCA). This protein contains a putative sequence for endoplasmic reticulum retention and all domains involved in calcium transport identified in mammalian SERCA. By immunofluorescence assays, an antibody against SERCA of E. histolytica detected EiSERCA in a vesicular network in the cytoplasm of E. invadens trophozoites, co-localizing with calreticulin. Interestingly, EiSERCA was redistributed close to plasma membrane during encystation, suggesting that this pump could participate in regulate the calcium concentration during this process. In addition, thapsigargin and cyclopiazonic acid, both specific inhibitors of SERCA, affected the number and structure of cysts, supporting the hypothesis that calcium flux mediated by SERCA has an important role in the life cycle of Entamoeba. |
| |
| Keywords: | Encystation SERCA Thapsigargin Cyclopiazonic acid Calcium homeostasis |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
