Increased oxidative stress and apoptosis in peripheral blood mononuclear cells of fructose-fed rats |
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| Affiliation: | 1. Department of Physiological Sciences, Federal University of Espírito Santo, Av Marechal Campos 1468, Maruipe, 29042-755 Vitória, ES, Brazil;2. Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha, ES, Brazil;1. Nihon University Graduate School of Dentistry at Matsudo, Removable Prosthodontics, Matsudo, Japan;2. Department of Removable Prosthodontics, Nihon University of Dentistry at Matsudo, Japan;3. Department of Dental Biomaterials, Nihon University of Dentistry at Matsudo, Japan;4. Department of Biomaterials & Biomimetics, New York University College of Dentistry, USA;1. Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria;2. Laboratory of Haematopathology and Immunology, National Specialised Hospital for Active Treatment of Haematological Diseases, Sofia, Bulgaria;3. Department of Immunology, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria;4. Department of Automation, Faculty of Automatics, Technical University of Sofia, Sofia, Bulgaria;5. Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria;1. Department of Immunology and Infectious Disease, John Curtin School of Medical Research, Australian National University, Canberra, Australia;1. Department of Pulmonary and Critical Care Medicine, Cleveland Clinic Florida, Weston, FL;2. Department of Thoracic and Cardiovascular Surgery, Cleveland Clinic Florida, Weston, FL;3. Department of Radiology, Cleveland Clinic Florida, Weston, FL;4. Department of Pulmonary Medicine, Respiratory Institute, Cleveland Clinic, Cleveland, OH;1. Division of Pathology, National Cancer Center Hospital, Tokyo, Japan;2. Division of Diagnostic Radiology, National Cancer Center Hospital, Tokyo, Japan;3. Division of Thoracic Surgery, National Cancer Center Hospital, Tokyo, Japan;4. Department of Thoracic Surgery, Juntendo University School of Medicine, Tokyo, Japan |
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| Abstract: | BackgroundMeasuring of oxidative stress in peripheral blood mononuclear cells is a suitable model of dietary induced systemic oxidative stress. Thus, we aimed to evaluate whether a chronic high fructose intake could induce oxidative damage in peripheral blood and bone marrow mononuclear cells of rats.MethodsAnimals were randomly assigned to the following groups: Control group (standard rat chow and tap water n = 8), and Fructose group (standard rat chow and a 10% fructose solution in the drinking water n = 8). Reactive oxygen species and cytokines were measure using flow cytometry in peripheral blood and bone-marrow mononuclear cells. Apoptotic cell death and the advanced oxidation protein products (AOPP) were also determined.ResultsWe observed a significant increase in ROS production in peripheral blood mononuclear cells of fructose group as compared to control rats. Apoptosis and the AOPP were higher in those animals underwent high fructose intake. Serum levels of IL-6 and IL-12 were also increased after 12 weeks of high fructose intake.ConclusionWe concluded that fructose intake leads to systemic oxidative stress and pro-inflammatory condition which affect peripheral blood mononuclear cells and bone-marrow mononuclear cells viability. |
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| Keywords: | Fructose Mononuclear cells Oxidative stress Apoptosis Inflammation |
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