A fast and effective method of quantitative analysis of VB1, VB2 and VB6 in B-vitamins complex tablets based on three-dimensional fluorescence spectra

Scattering signals and partial overlapping of target peaks usually appear in fluorescence determination. In this study, the improved wavelet moment method was proposed and applied to the quantitative determination of multiple target components in real B-vitamins complex tablets based on three-dimensional (3D) fluorescence spectra. The correlation coefficients (R) were more than 0.9939, and the correlation coefficients of leave-one-out cross-validation (R_LOO-cv) were more than 0.9376. The inter-day and intra-day variations were less than 5.8% and 6.1%, respectively. The recovery ranged from 97.6% to 109.4%. The limits of detection (LODs) and quantification (LOQs) were less than 0.07 μg mL⁻¹ and 0.13 μg mL⁻¹, respectively. All the statistical parameters indicated that the method was stable and reliable. The application to the practical samples confirmed that wavelet moment method effectively extracted the features of target components in 3D spectra and the proposed method could be applied to the analyses of food or other complex samples.