Biocatalytic and antibacterial visualization of green synthesized silver nanoparticles using Hemidesmus indicus |
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| Affiliation: | 1. Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India;2. Department of Zoology, University of Madras, Guindy campus, Chennai 600 025, India;3. Department of Nanoscience and Technology, Alagappa University, Karaikudi 630 004, India;1. Department of Biophysics, Faculty of Dentistry, University of Buenos Aires, Argentina;2. Department of Pharmacology, Faculty of Dentistry, University of Buenos Aires, Argentina;1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;2. School of Basic Medical Sciences of Lanzhou University, Lanzhou 730000, China;3. General Hospital of Lanzhou Petrochemical Company, Lanzhou 730060, China;4. Evidence-based Medicine Center of Lanzhou University, Lanzhou 730000, China;1. Department of Periodontology, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan;2. Department of Microbiology, Tokyo Dental College, 2-1-14 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan;3. Panasonic Healthcare, 2-38-5 Nishishinbashi, Minato-ku, Tokyo 105-8433, Japan;4. Department of Clinical Oral Health Science, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan;5. Oral Health Science Center, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan;1. College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People''s Republic of China;2. Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People''s Republic of China;1. Department of Dermatology, Lianyungang First People''s Hospital, Lianyungang 222002, China;2. Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China |
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| Abstract: | In the present investigation, we described the green synthesis of silver nanoparticles using plant leaf extract of Hemidesmus indicus. The synthesized silver nanoparticles were characterized by UV–visible spectroscopy, fourier transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX). TEM images proved that the synthesized silver nanoparticles were spherical in shape with an average particle size of 25.24 nm. To evaluate antibacterial efficacy, bacteria was isolated from poultry gut and subjected to 16S rRNA characterization and confirmed as Shigella sonnei. The in vitro antibacterial efficacy of synthesized silver nanoparticles was studied by agar bioassay, well diffusion and confocal laser scanning microscopy (CLSM) assay. The H. indicus mediated synthesis of silver nanoparticles shows rapid synthesis and higher inhibitory activity (34 ± 0.2 mm) against isolated bacteria S. sonnei at 40 μg/ml. |
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| Keywords: | Silver nanoparticles Biosynthesis CLSM |
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