Identification of environmental chemicals that induce yolk malabsorption in zebrafish using automated image segmentation |
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| Affiliation: | 1. Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA;2. Department of Computer Science, University of Houston, Houston, TX 77204, USA;3. Karolinska Institutet, Department of Biosciences and Nutrition, 14183 Huddinge, Sweden;1. University of Houston, Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, Houston, TX 77204, USA;2. Karolinska Institutet, Department of Biosciences and Nutrition, 14183 Huddinge, Sweden;3. North Carolina State University, Department of Biological Sciences, Bioinformatics Research Center, Raleigh, NC 27695-7614, USA;1. Zebrafishlab, Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium;2. Systemic Physiological and Ecotoxicological Research (SPHERE), Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium;1. Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden;2. Environmental Chemistry Unit, Department of Materials and Environmental Chemistry, Stockholm University, SE-106 91 Stockholm, Sweden;1. Rutgers, The State University of New Jersey, Department of Environmental Sciences, New Brunswick, NJ, USA, USA;2. Rutgers, The State University of New Jersey, Joint Graduate Program in Toxicology, New Brunswick, NJ, USA, USA |
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| Abstract: | Environmental factors affecting nutrient availability during development can cause predisposition to diseases later in life. To identify chemicals in the environment capable of altering nutrient mobilization, we analyzed yolk malabsorption in the zebrafish embryo, which relies on maternally-derived yolk for nutrition during its first week of life. Embryos of the transgenic zebrafish line HGn50D, which fluoresce in the yolk syncytial layer, were exposed from two to five days post fertilization to different chemicals. We developed a software package to automatically and accurately segment and quantify the area of the fluorescing yolk in images captured at the end of the treatment period. Based on this quantification, we found that prochloraz decreased yolk absorption, while butralin, tetrabromobisphenol A, tetrachlorobisphenol A and tributyltin increased yolk absorption. Given the number and variety of industrial chemicals in commerce today, development of automated image processing to perform high-speed quantitative analysis of biological effects is an important step for enabling high throughput screening to identify chemicals altering nutrient absorption. |
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| Keywords: | Zebrafish Yolk Toxicity Image analysis High throughput screening |
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