Outer membrane vesicles isolated from two clinical Acinetobacter baumannii strains exhibit different toxicity and proteome characteristics |
| |
| Affiliation: | 1. Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, Córdoba, Spain;2. Red Española de Investigación en Patología Infecciosa (REIPI), Instituto de Salud Carlos III, Madrid, Spain;3. Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA;4. Unidad de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Instituto de Biomedicina de Sevilla/Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Sevilla, Spain;5. Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, Córdoba, Spain;6. Departamento de Botánica, Ecología y Fisiología Vegetal, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, Córdoba, Spain;7. Departamento de Química Analítica, Universidad Complutense de Madrid, Madrid, Spain;8. Sección de Enfermedades Infecciosas Pediátricas e Inmunopatología, Hospital Universitario Infantil Virgen del Rocío, Sevilla, Spain;9. Department of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY, USA |
| |
| Abstract: | Outer membrane vesicles (OMVs) are well-characterized virulence factors produced by Gram-negative bacteria. Here, we isolated two clinical Acinetobacter baumannii strains, the multidrug-resistant A. baumannii (MDRAb) A38 and non-MDRAb 5806. Strain A38 produced more abundant OMVs than strain 5806 when cultured to the early stationary phase. The results from cell proliferation assays and real-time PCR analyses indicated that A38 OMVs induced more powerful cytotoxicity and stronger innate immune responses compared with 5806 OMVs. Moreover, SDS-PAGE and LC-MS/MS analyses revealed that A38 OMVs contained more virulence factors, including Omp38, EpsA, Ptk, GroEL, hemagglutinin-like protein, and FilF. Taken together, the results of the present study suggest that MDRAb might produce abundant OMVs with more virulent factors facilitating the worse outcome, a finding that merits further study. |
| |
| Keywords: | Outer membrane vesicles Virulence Innate immunity Proteomics MDRAb" },{" #name" :" keyword" ," $" :{" id" :" kwrd0040" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" __text__" ," _" :" multidrug resistant " },{" #name" :" italic" ," _" :" Acinetobacter baumannii OMVs" },{" #name" :" keyword" ," $" :{" id" :" kwrd0050" }," $$" :[{" #name" :" text" ," _" :" outer membrane vesicles PAMPs" },{" #name" :" keyword" ," $" :{" id" :" kwrd0060" }," $$" :[{" #name" :" text" ," _" :" pathogen-associated molecular patterns MLST" },{" #name" :" keyword" ," $" :{" id" :" kwrd0070" }," $$" :[{" #name" :" text" ," _" :" multilocus sequence typing |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
