Outer membrane vesicles isolated from two clinical Acinetobacter baumannii strains exhibit different toxicity and proteome characteristics |
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| Institution: | 1. Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, Córdoba, Spain;2. Red Española de Investigación en Patología Infecciosa (REIPI), Instituto de Salud Carlos III, Madrid, Spain;3. Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA;4. Unidad de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Instituto de Biomedicina de Sevilla/Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Sevilla, Spain;5. Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, Córdoba, Spain;6. Departamento de Botánica, Ecología y Fisiología Vegetal, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, Córdoba, Spain;7. Departamento de Química Analítica, Universidad Complutense de Madrid, Madrid, Spain;8. Sección de Enfermedades Infecciosas Pediátricas e Inmunopatología, Hospital Universitario Infantil Virgen del Rocío, Sevilla, Spain;9. Department of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY, USA |
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| Abstract: | Outer membrane vesicles (OMVs) are well-characterized virulence factors produced by Gram-negative bacteria. Here, we isolated two clinical Acinetobacter baumannii strains, the multidrug-resistant A. baumannii (MDRAb) A38 and non-MDRAb 5806. Strain A38 produced more abundant OMVs than strain 5806 when cultured to the early stationary phase. The results from cell proliferation assays and real-time PCR analyses indicated that A38 OMVs induced more powerful cytotoxicity and stronger innate immune responses compared with 5806 OMVs. Moreover, SDS-PAGE and LC-MS/MS analyses revealed that A38 OMVs contained more virulence factors, including Omp38, EpsA, Ptk, GroEL, hemagglutinin-like protein, and FilF. Taken together, the results of the present study suggest that MDRAb might produce abundant OMVs with more virulent factors facilitating the worse outcome, a finding that merits further study. |
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| Keywords: | Outer membrane vesicles Virulence Innate immunity Proteomics MDRAb"} {"#name":"keyword" "$":{"id":"kwrd0040"} "$$":[{"#name":"text" "$$":[{"#name":"__text__" "_":"multidrug resistant "} {"#name":"italic" "_":"Acinetobacter baumannii OMVs"} {"#name":"keyword" "$":{"id":"kwrd0050"} "$$":[{"#name":"text" "_":"outer membrane vesicles PAMPs"} {"#name":"keyword" "$":{"id":"kwrd0060"} "$$":[{"#name":"text" "_":"pathogen-associated molecular patterns MLST"} {"#name":"keyword" "$":{"id":"kwrd0070"} "$$":[{"#name":"text" "_":"multilocus sequence typing |
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