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| 阿司匹林、舒林酸对3AO细胞环氧合酶-2、血管生长因子、层粘蛋白受体、CD44V6表达的影响 | |
| 引用本文： | 高雪芹,韩金祥,张维东,宋守芹,朱波. 阿司匹林、舒林酸对3AO细胞环氧合酶-2、血管生长因子、层粘蛋白受体、CD44V6表达的影响[J]. 中国临床药理学与治疗学, 2004, 9(8): 906-910 |
| 作者姓名： | 高雪芹 韩金祥 张维东 宋守芹 朱波 |
| 作者单位： | 1. 卫生部生物技术药物重点实验室,山东省医药生物技术研究中心,济南,250062;山东山东大学医学院,济南,250014,山东 2. 山东省医学科学院基础医学研究所,济南,250062,山东 3. 卫生部生物技术药物重点实验室,山东省医药生物技术研究中心,济南,250062,山东 |
| 基金项目： | 山东省卫生厅青年基金资助项目 (№ 1999CA2CACA1) |
| 摘 要： | 目的：研究阿司匹林、舒林酸对3AO细胞环氧合酶-2(COX-2)、血管生长因子(VEGF)、CD44V6及层粘蛋白受体(LN—R)表达的影响：方法：采用MTF方法观察阿司匹林、舒林酸在贴壁前后加入培养液中对3AO细胞生长的影响：采用免疫细胞化学的方法研究两种药物对3AO细胞COX-2、VEGF、CD44V6、LN—R的影响。结果：MTT实验显示阿司匹林、舒林酸不论在贴壁前还是后加入均可抑制3AO卵巢癌细胞的生长，加药后调节pH可减弱生长抑制作用但仍然可明显抑制肿瘤细胞的生长：阿司匹林和舒林酸对COX-2、VEGF的表达也有抑制作用，但对CD44V6及LN-R的表达无明显影响。结论：抑制COX-2的表达可以下调VEGF的表达，从而抑制肿瘤的血管生成，阿司匹林和舒林酸可通过抑制COX-2及VEGF的表达而抑制肿瘤的生长。 |
| 关 键 词： | 阿司匹林 舒林酸 COX-2 VEGF CD44V6 LN-R |
| 文章编号： | 1009-2501(2004)08-0906-05 |
| 修稿时间： | 2004-05-26 |
Effects of aspirin and sulindac on expression of cyclooxygenase-2, vascular endothelial growth factor, laminin receptor, and CD44V6 in 3AO cells |
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| GAO Xue-Qin ,,HAN Jin-Xiang ,,ZHANG Wei-Dong,SONG Shou-Qin,ZHU Bo Shandong Medicinal and Biotechnology Center,Shandong Academy of Medical Sciences,Key Laboratory for Biotech-drug,Ministry of Health,Jinan ,Shangdong,China, Medical College of Shandong University,Jinan ,Shangdong,China. Effects of aspirin and sulindac on expression of cyclooxygenase-2, vascular endothelial growth factor, laminin receptor, and CD44V6 in 3AO cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(8): 906-910 | |
| Authors: | GAO Xue-Qin HAN Jin-Xiang ZHANG Wei-Dong SONG Shou-Qin ZHU Bo Shandong Medicinal Biotechnology Center Shandong Academy of Medical Sciences Key Laboratory for Biotech-drug Ministry of Health Jinan Shangdong China Medical College of Shandong University Jinan Shangdong China |
| Affiliation: | GAO Xue-Qin 1,2,HAN Jin-Xiang 1,2,ZHANG Wei-Dong3,SONG Shou-Qin3,ZHU Bo1 1Shandong Medicinal and Biotechnology Center,Shandong Academy of Medical Sciences,Key Laboratory for Biotech-drug,Ministry of Health,Jinan 250062,Shangdong,China, 2Medical College of Shandong University,Jinan 250014,Shangdong,China, 3Institute of Basic Medicine,Shandong Academy of Medical Sciences,Jinan 250062,Shangdong,China |
| Abstract: | AIM: To investigate the effects of aspirin and sulindac on the expression of cyclooxygenase-2 (COX-2), vascular endothelial growth factor (VEGF), CD 44V 6 and laminin receptor (LN-R) in 3AO cells. METHODS: The growth inhibition effects of aspirin and sulindac in 3AO cells were tested by MTT assay. The expression of COX-2, VEGF, LN-R and CD 44V 6 were evaluated by immunocytochemistry. RESULTS: Aspirin and sulindac significantly inhibited the growth of 3AO cells whether added before or after cells adhered. When the pH was adjusted with sodium bicarbonate, the inhibition had a little decrease. Aspirin and sulindac also inhibited the expression of COX-2 and VEGF. But there were no effect on the expression of LN-R and CD 44V 6. CONCLUSION: Inhibited expression of COX-2 may down-regulate the expression of VEGF, and inhibit the angiogenesis of tumor. Aspirin and sulindac may exert their chemopreventive and anticancer effects by inhibiting the expressions of COX-2 and VEGF. |
| Keywords: | aspirin sulindac cyclooxygenase-2 vascular endothelial growth factor laminin receptor CD_(44)V_6 |
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