| 高效液相色谱法测定芩连片中连翘苷含量 |
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| 引用本文： | 袁明,赵协慧.高效液相色谱法测定芩连片中连翘苷含量[J].辽宁中医药大学学报,2012(2):173-174. |
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| 作者姓名： | 袁明 赵协慧 |
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| 作者单位： | 青海大学医学院 |
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| 摘 要： | 目的:建立芩连片中连翘苷的含量测定方法。方法:采用高效液相色谱法,色谱柱为Kromasil C18(250mm×4.6mm,5μm),流动相为乙腈-水(25∶75,v/v),流速为1.0 mL.min-1,检测波长为277nm。结果:连翘苷在0.375~2.250μg范围内呈良好的线性关系(r=0.9998),平均回收率为97.4%,RSD为1.30%。结论:本方法灵敏、准确、专属性强,可用于芩连片中连翘苷的含量测定。
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| 关 键 词： | 芩连片 连翘苷 高效液相色谱法 |
Determination of Phillyrin in Qinlian Tablets by HPLC |
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| YUAN Ming,ZHAO Xie-hui.Determination of Phillyrin in Qinlian Tablets by HPLC[J].Journal of Liaoning University of Traditional Chinese Medicine,2012(2):173-174. |
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| Authors: | YUAN Ming ZHAO Xie-hui |
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| Institution: | (Medical College of Qinghai University,Xining 810001,Qinghai,China) |
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| Abstract: | Objective:To establish a method for determination of phillyrin in Qinlian tablets.Methods:HPLC analysis was carried out on Kromasil C18 column(250mm×4.6mm,5μm)and the mixture of acetonitrile-water(25∶75,v/v)was used as the mobile phase,the flow rate was 1.0mL·min-1 and the detection wavelength was at 277nm.Results:The standard curve was linear within the range of 0.375~2.250μg(r = 0.9998),the average recovery was 97.4 %,RSD was 1.30%.Conclusion:The method is semitive,accurate,specific and can be used for the determination of phillyrin in Qinlian tablets. |
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| Keywords: | Qinlian tablets phillyrin HPLC |
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