| 退白汤中补骨脂素和异补骨脂素含量测定方法研究 |
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| 引用本文： | 盛国荣.退白汤中补骨脂素和异补骨脂素含量测定方法研究[J].辽宁中医药大学学报,2012(2):11-12. |
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| 作者姓名： | 盛国荣 |
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| 作者单位： | 江苏省南通市皮肤病性病防治所 |
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| 基金项目： | 南通市指令性社会发展科技计划资助项目(S2009047) |
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| 摘 要： | 目的:建立双波长薄层扫描法测定退白汤中补骨脂素和异补骨脂素含量的方法。方法:选用补骨脂素λS=272nm,λR=305nm;异补骨脂素λS=275nm,λR=310nm;以甲苯-乙酸乙酯-甲酸(15:2:1)为展开剂,并在365nm紫外灯下检视。结果:补骨脂素在0.27~1.62μg呈良好的线性关系(γ=0.9987),平均回收率99.46%,RSD=1.34%(n=5);异补骨脂素在0.25~1.50μg呈良好的线性关系(γ=0.9992),平均回收率99.60%,RSD=1.37%(n=5)。结论:本法简便、快捷,可用于退白汤中补骨脂素和异补骨脂素的含量测定。
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| 关 键 词： | 退白汤 双波长薄层扫描法 补骨脂素 异补骨脂素 |
Determination Method of Psoralen and Isopsoralen for Anti-vitiligo Decoction |
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| SHENG Guo-rong.Determination Method of Psoralen and Isopsoralen for Anti-vitiligo Decoction[J].Journal of Liaoning University of Traditional Chinese Medicine,2012(2):11-12. |
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| Authors: | SHENG Guo-rong |
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| Institution: | SHENG Guo-rong(Nantong Institution for Dermatosis and Venereal Disease Prevention,Nantong 226006,Jiangsu,China) |
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| Abstract: | Objective:To establish the determination method of dual-wavelength TLC for psoralen and isopsoralen in Anti-vitiligo decoction.Methods:choose λS=272nm,λR=305nm for determining psoralen,and choose λS=275nm,λR=310nm for determining isopsoralen.Developing agent:Toluene-ethyl acetate-formic acid(15:2:1),and determined under 35nm UV light.Results:Psoralen is under good linear relationship in the range of 0.27~1.62μg(γ=0.9987),and the average recovery ratio is 99.46%,RSD=1.34%(n=5).Isopsoralen is under good linear relationship in the range of 0.25~1.5μg(γ=0.9992),and the average recovery ratio is 99.60%,RSD=1.37%(n=5).Conclusions:The method is simple and fast for the determination of psoralen and isopsoralen for Anti-vitiligo decoction. |
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| Keywords: | Anti-vitiligo decoction dual-wavelength TLC method psoralen isopsoralen |
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