| 肺癌EB病毒LMP1基因片段的检测及变异株的意义初探 |
| |
| 引用本文： | 王宝梅,银平章,彭晓帆,孔令非. 肺癌EB病毒LMP1基因片段的检测及变异株的意义初探[J]. 实用诊断与治疗杂志, 2002, 16(2): 96-97 |
| |
| 作者姓名： | 王宝梅 银平章 彭晓帆 孔令非 |
| |
| 作者单位： | 450003,郑州市,河南省人民医院病理科 |
| |
| 基金项目： | 该课题为河南省自然科学基金项目(994025500) |
| |
| 摘 要： | 目的研究EB病毒LMP1基因与肺癌的关系,并对其变异株进行分析.方法应用多聚酶链反应检测了40例肺癌EBV-LMP1基因片段,并对其进行克隆及测序分析.结果40例肺癌标本中,11例(27.5%)阳性;对11例EBV-LMP1 DNA阳性产物用限制性内切酶StyⅠ酶切消化,发现9例与标准病毒完全一致,2例不同,提示EBV-LMP1基因有变异存在.结论EB病毒的感染及LMP1基因片段的变异在肺癌的发生、发展中可能发挥着一定的作用.
|
| 关 键 词： | 肺肿瘤 疱诊病毒4型 人 基因克隆 核苷酸序列测定 |
| 修稿时间： | 2001-08-19 |
Detection of EB virus LMP1 fragment and the preliminary exploration of the significance of its mutation in pulmonary carcinoma |
| |
| WANG Baomei.YIN Pingzhang,PENG Xiaofan,et al.. Detection of EB virus LMP1 fragment and the preliminary exploration of the significance of its mutation in pulmonary carcinoma[J]. Journal of Practical Diagnosis and Therapy, 2002, 16(2): 96-97 |
| |
| Authors: | WANG Baomei.YIN Pingzhang PENG Xiaofan et al. |
| |
| Affiliation: | WANG Baomei.YIN Pingzhang,PENG Xiaofan,et al. Department of Pathology. Henan Provincial People's Hospital,Zhengzhou 450003,China |
| |
| Abstract: | ve To study the relationship between EB virus LMP1 fragment and pulmonary carcinoma and assess the significance of the mutation. Methods EBV- LMPl fragments were amplified in 40 cases of pulmonary carcinoma by PCR and then cloned and sequenced. Results In 40 cases of pulmonary carcinoma, 11 cases (27. 5%) were positive. These 11 cases of positive products were then analyzed with restrictive enzyme sty I . Among them, 9 cases were the same with standard EBV DNA.and the other 2 cases existed mutation. Conclusion The EBV infection and the mutation of LMP1 fragment may play an important role in the genesis and development of pulmonary carcinoma. |
| |
| Keywords: | Lung neoplasm herpesvirus 4 human gene cloning DNA sequencing |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
