暗适应时间对SD大鼠视觉电生理的影响

目的 研究暗适应时间对SD大鼠视觉电生理检查结果的影响，用于制定标准化实验记录方案，便于比较不同实验室间的结果。方法 实验研究。42只SD大鼠（42眼）随机分为7组，分别于暗适应15 min、30 min、1 h、4~6 h、12~14 h、15~17 h、24~26 h后记录视觉电生理。采用单因素方差分析比较各组间差异，post hoc检验分析两两间差异。结果 暗适应时间对大鼠视觉电生理的影响主要发生于暗适应视网膜电图a波和b波的潜伏期，表现为15 min暗适应时间组较其余各组潜伏期长（P<0.05）；30 min以上暗适应时间，各组潜伏期差异无统计学意义。结论 暗适应时间影响视网膜电图（ERG）记录，对SD大鼠暗适应ERG记录的暗适应时间不宜少于30 min。

Effects of the duration of dark adaptation on the retinal function of normal SD rats

Objective To study the effect of the duration of dark adaptation on electroretinography （ERG） in SD rats； to develop standardized research programs so results can easily be compared between different laboratories. Methods In an experimental study， 42 SD rats （42 eyes） were randomly divided into 7 groups based on the duration of dark adaptation： 15 min， 30 min， 1 h， 4-6 h， 12-14 h， 15-17 h， and 24-26 h. Visual electrophysiology recordings were then taken. ANOVA was used to analyze the differences among the groups and a post hoc analysis was used to compare the difference between any two groups. Results Different dark adaptation durations mainly affected the latencies of the dark-adapted ERG a-wave and b-wave of the rats′ ERG. The latency of 15 min dark adaption time was delayed longer compared with other dark adaption time group （P<0.05）. There was no significant effect when dark adaptation time was longer than 30 minutes. Conclusion Dark adaptation durations impact ERG recordings. The minimum dark adaptation time for rat electroretinography recording is 30 minutes.