款冬花紫菀配伍对绿原酸在大鼠体内药代动力学影响

目的：研究款冬花、紫菀配伍对绿原酸在大鼠体内药代动力学参数的影响。方法：在筛选款冬花止咳有效部位、紫菀止咳增效部位的基础上，将SD大鼠随机分成4组，分别灌胃给予绿原酸单体、款冬花部位、紫菀部位、款冬花部位＋紫菀部位（即部位配伍），采用HPLC法测定血浆中的绿原酸浓度，并用WinNonlin药动学软件根据非房室模型法计算药动学参数。结果：大鼠灌胃绿原酸单体、款冬花部位、紫菀部位、部位配伍后的主要药动参数：AUC0-t分别为245．09±14．05，349．27±62．15，105．11±18．57，496．77±77．75μg·mL^-1·min^-1；t1/2分别为323．70±51．00，464．53±126．10，408．27±31．30，203．02±7．12min；Cmax分别为1．05±0．13，1．52±0．55，0．63±0．04，4．49±0．59μg·mL^-1；Tmax分别为60．00±0．00，15．00±0．00，15．00±0．00，15．00±0．00min。结论：紫菀能促进大鼠对款冬花中绿原酸的吸收，加快绿原酸的消除，减少绿原酸在体内的蓄积，可以部分诠释款冬花、紫菀“相须配伍”增效的机理。

Pharmacokinetics of Chlorogenic Acid in Rats on Compatibility of Extracts of Farfarae Flos and Asteris Radix

Objective：To study the pharmacokinetic features of chlorogenic acid before and after the combination of the extracts of Farfarae Flos and Asteris Radix in rats. Methods ： SD rats were randomized into 4 groups and were intragastric administered with chlorogenic acid （CA） , Farfarae Flos extract （FF）, Asteris Radix extract （AR） and the combined extract of FF＋AR, respectively. A HPLC method was established to determine chlorogenic acid in rat plasma. The main pharmacokinetic parameters were calculated with WinNonlin software by non-compartmental methods. Results： The main pharmacokinetic parameters of chlorogenic acid in 4 groups of CA, FF, AR and FF＋AR were as follows in order： AUC （μg·mL^-1·min^-1） were 245.09± 14.05, 349.27±62. 15, 105.11±18.57, 496.77±77.75; t1/2 （min） were 323.70±51.00, 464. 53±126.10, 408.27±31.30, 203.02±7.12; Cmax （μg·mL^-1） were 1.05±0.13, 1.52±0.55, 0.63±0.04, 4.49±0.59; Tmax （min） were 60. 00±0.00, 15.00±0.00, 15.00±0.00, 15.00±0.00. Conclusion ： The synergy of compatibility of Farfarae Flos and Asteris Radix could be explained by enhancing the absorption and accelerating the elimination of chlorogenic acid in rat plasma.