金菊颗粒质量标准研究

目的：建立金菊颗粒质量研究标准。方法：采用薄层色谱法对金菊颗粒中的菊花、连翘、赤芍和黄芩进行定性鉴别；采用高效液相色谱法测定制剂中绿原酸的含量。色谱柱：Phenomenex C18（250mm×4．6mm，5μm）；流动相：甲醇-醋酸溶液水-冰醋酸-三乙胺（85：1：0．3）l（17：83）；检测波长：327nm；流速：0．8mL／min；柱温：30℃。结果：大黄、黄芩、赤芍、连翘和菊花特征斑点清晰，分离度好，阴性对照无干扰。绿原酸在250．8～1504．8μg范围内呈良好的线性关系，平均回收率为98．25％，RSD为0．93％（n=9）。结论：该方法简便准确、专属性强、重复性好，可作为金菊颗粒的质量控制标准。

Study on Quality Standard of Jinju Granules

Objective：To establish a method for quality control of Jinju granules. Methods：Chrysanthemi Flos, Forsythiae Fruc tus, Paeoniae Radix Rubra and Scutellariae Radix in Jinju granules were indetified by thin-layer chromatography. The content of Chlorogenic acid was determined by HPLC. The column was Phenomenex C18 （250mm×4.6mm,5μm） , the mobile phase was methanol-ethylic acid solution water-acetic acid-triethylamine （85 ： 1 ： 0.3）]（17 ： 83）. The flow rate was 0.8mL/min and column temperature was 30℃. The detective wavelength was 327nm. Results：The spots on TLC plates were clear without interference in the blank reference. The linearly range of chlorogenic acid was 250.8-1 504.8μg. The average recovery of chlorogenic acid was 98. 25% and RSD was 0.93% （n=9）. Conclusion： The method is simple, rapid and accurate, and it can be adopted for the quality control of Jinju granules and content determination of chlorogenic acid.