视觉刺激状态下正常成人电针光明穴的脑功能性磁共振研究

目的:对比研究针刺足少阳胆经光明穴(GB37)与视觉刺激后引起的人脑血液动力学反应,探讨分析视觉刺激状态下针刺对脑功能活动区的影响。方法:25例健康志愿者随机分为无视觉刺激电针组(10例)与视觉刺激状态电针组(15例)。视觉刺激状态电针组15例健康自愿者中12例取得成功,并在2种不同的刺激模式(包括视觉刺激任务与视觉刺激状态下针刺光明穴)状态下进行血氧水平依赖性功能磁共振(BOLD-fMRI)实验;应用闪烁棋盘方格为视觉刺激任务,频率8Hz,最后以图像数据重组方法,检测受试者在接受视觉刺激任务与针刺状态时的大脑响应功能区的激活,并进行分析比较。结果:视觉刺激任务时见脑干、小脑后叶大面积激活、及边缘叶、海马、颞中回、颞下回激活;针刺状态时见顶叶中央后回、顶下小叶、顶叶楔前叶、额中回激活。在视觉刺激下时电针光明穴见额上回,额下回、顶叶楔前叶及枕叶舌回激活。结论:功能性磁共振技术可看出针刺后脑功能响应功能区的激活情况;视觉刺激的参与导致针刺脑激活区改变,且该变化具有重要的意义;针刺足少阳胆经光明穴对视觉系统转导通路整合有一定影响;此外,外界的刺激亦对针刺疗效可能有着显著的影响。

Cerebral fMRI study of the normal adult on electro-acupuncture Guangming point (GB37) under the vision stimulation condition

AIM: To study the human brain hemodynamics responses induced by acupuncturing foot shaoyang gallbladder meridian point GB37 and visual stimulation, and to analyze the effect of acupuncture to activate the cerebral functional area in visual stimulus. METHODS: Twenty five normal subjects were divided into two groups: 15 subjects for electro-acupuncture GB37 in visual stimulus group and 10 subjects for electro-acupuncture GB37 groups. The blood oxygen level dependent functional magnetic resonance imaging (BOLD-fMRI) was carried out under the condition of two different stimulation patterns (including a vision stimulation task and point GB37 acupuncture under the vision stimulation condition). A scintillating chessboard grid was applied as the vision stimulation task and the frequency was 8 Hz. The activation of cerebral functional areas of tested people under the conditions of the vision stimulation task and acupuncture were analyzed, respectively. RESULTS: Out of 15 subjects of electro-acupuncture GB37 in visual stimulus groups, twelve health volunteers were enrolled. When the vision stimulation task was carried out, the alarge area of brainstem and posterior lobe of cerebellum was seen to be activated. The areas of limbic lobe, hippocampus, middle temporal gyrus and inferior temporal gyrus were also activated. Under the condition of acupuncture only, the areas of posterior central gyrus, inferior parietal lobule, parietal lobe precuneus and the middle frontal gyrus were activated. When double-sided points of gallbladder meridian of foot-shao-yang were electro-acupunctured under the visual stimulus status, the areas of superior frontal gyrus, inferior frontal gyrus, parietal precuneus and occipital gyrus lingualis were activated. CONCLUSION: The functional MRI shows the activation of cerebral functional area after acupuncture. Visual stimulus causes significant changes of brain conduction path connection. Under the visual stimulus status, the acupuncture to the points of gallbladder meridian of foot-shao-yang, GB37 has an extent of influence to conduction path conformity of the vision system and with the treatment of ophthalmocace. Furthermore, the external stimulation has prominent influence to the acupuncture therapy possibly.