乳腺导管上皮病变中细胞增殖和凋亡作用及其与ER、PR、C-erbB-2表达关系的研究

目的 :探讨乳腺导管上皮病变中细胞增殖和凋亡作用及其与ER、PR、C -erbB - 2表达关系的临床病理意义。方法 :采用免疫组织化学Envision二步法和TUNEL法检测正常乳腺导管上皮 2 0例 ,增生性乳腺导管上皮 4 6例 ,导管原位癌 12例和 6 0例乳腺浸润性导管癌增殖指数 (ProliferativeIndex ,PI)和凋亡指数 (ApoptoticIn dex ,AI)以及ER、PR、C -erbB - 2表达 ,分析乳腺导管上皮病变中细胞增殖指数和凋亡指数及其与ER、PR、C-erbB - 2表达率的关系。结果 :正常乳腺导管上皮、增生性导管上皮、导管原位癌、浸润性导管癌及其随病理组织学分级愈高 ,细胞增殖指数 (PI)和C -erbB - 2阳性表达指数 (CI)逐渐增加 ,细胞凋亡 (AI)逐渐减少 ,且正常乳腺导管上皮与乳腺导管原位癌 (重度非典型增生 )细胞增殖指数 (PI)、细胞凋亡 (AI)、C -erbB - 2阳性表达 (CI)及ER、PR表达 ,差异有显著性 (P <0 0 5 )。乳腺导管上皮病变细胞增殖与ER、C -erbB - 2表达呈正相关 (r=0 5 4 ,r=0 79,P <0 0 5 ) ;乳腺导管上皮病变细胞凋亡与ER、C -erbB - 2表达呈负相关 (r =-0 6 2 ,r=- 0 73,P <0 0 5 )。结论 :细胞增殖和凋亡、ER、PR及C -erbB - 2在乳腺导管上皮病变发生发展和预后判断过程中可能起重要作用 ,且它们皆可能作为乳

The Studv of the Relationships between Cell Proliferation and Apoptosis and ER, PR, C - erbB - 2 Expression in Breast Duct Epithelium Lesions

Objective:To investigate the role of cell proliferation and apoptosis and the relationships between proliferation and apoptosis and ER?PR?C-erbB-2 expressions in breast duct epithelium lesions.Methods:The methods of Envision and TUNEL were used to investigate the occurrence of cell proliferation and apoptosis and ER?PR?C-erbB-2 expressions in 138 cases breast duct epithelium lesions and the relationships between proliferation and apoptosis and ER?PR?C-erbB-2 expression were analyzed.Results:Cell proliferation index(PI)and C-erbB-2 expression(CI)were gradually increase from normal breast duct epithelium,benign proliferation to carcinoma,but the apoptosis index(AI)was gradually decreasing.There was a striking significance(P<0.05)for the expression of PI,AI,CI,ER and PR between the normal breast duct epithelium and carcinoma in situ.There was a significant direct correlation between cell proliferation and ER and C-erbB-2 expressions in breast duct epithelium lesions(r=0.54,r=0.79,P<0.05),but apoptosis index(AI) were negative(r=-0.62,r=-0.73,P<0.05).Conclusions:The cell proliferation,apoptosis,ER,PR and C-erbB-2 expressions might play important roles in the pathogenesis and prognosis of breast duct epithelium lesions,and detecting their expressions might be a useful markers in judging early malignant change of breast duct epithelium.