GSH在H2O2致DNA损伤中的作用研究

目的：研究还原型谷胱苷肽（GSH）对H2O2致DNA损伤的影响。方法：应用新型彗星图象分析系统以及单细胞凝胶电泳（SCGE）。结果：应用该系统检测了GSH在H2O2致DNA损伤的作用，尾长、尾重心、Olive尾矩、尾惯量、尾分布矩、尾DNA％以及尾／头比等指标与H2O2浓度存在明显的剂量－效应关系，发现10μmol/L GSH能抑制H2O2的DNA断裂能力。结论：GSH有抑制H2O2致DNA损伤的作用，活性氧是DNA损伤的重要原因。

STUDY ON EFFECT OF GSH ON DNA DAMAGE DUE TO H2O2

Objective:To study the effect of GSH on DNA damage due to H 2O 2.Methods:Single cell gel electrophoresis was used. Results:Inhibition from GSH to damage on single strand DNA of H 2O 2 in peripheral lymphocyte was detected.There were dose-response relationship between H 2O 2 and indexes including length of tail,tail mean,tail Olive moment,tail inertia,tail distributed moment,tail DNA percentage and ratio of tail/head.Conclusion:GSH could inhibit damage on DNA due to H 2O 2.It suggested ROS can induce DNA damage.