玻璃体内注射曲安奈德对兔眼的毒性作用

目的观察玻璃体内注射曲安奈德对兔角膜、晶状体、睫状体和视网膜的毒性作用。方法32只灰兔分为四组(n=8),玻璃体内分别注射乳酸林格氏缓冲液(对照组)、4 mg曲安奈德(B组)、1.3 mg曲安奈德(C组)和载体(D组)。于注射前和注射后不同时间点分别进行眼压及明、暗适应视网膜电图检查;于术后1周、1月和3月,每组分别取2眼观察组织学和超微结构的改变。结果与其他各组比较,B组注射后1 d和1、2周的眼压显著上升(P<0.05)。术后1周和1月,B组和D组视网膜电图各峰值较对照组和C组明显下降(P<0.05)。光镜下B组视网膜组织受损;电镜下B组和D组的睫状体、晶状体和视网膜组织均不同程度受损。结论玻璃体内注射曲安奈德和载体后,对兔晶状体、睫状体和视网膜组织可能有一定的毒性作用。

Ocular toxicity of intravitreal injection of triamcinolone acetonide in rabbit eyes

Objective To evaluate the toxicity of intravitreal injection of triamcinolone acetonide on the cornea,lens,ciliary body and retina in rabbit eyes. Methods Thirty-two gray rabbits were divided into four groups(n=8).The rabbits were intravitreally injected with buffered saline solution(control group),4 mg triamcinolone acetonide(group B),1.3 mg triamcinolone acetonide(group C) and vehicle(group D).Intraocular pressure,scotopic and photopic electroretinogram examinations were performed before injection and at different time points after injection.Histologic and ultrastructural changes were observed 1 week,1 month and 3 months after injection. Results Compared with the other groups,the intraocular pressure of group B was significantly increased on day 1,week 1 and week 2 after injection(P<0.05).The amplitudes of electroretinogram of group B and D were significantly decreased than those of control group and group C on week 1 and month 1 after injection(P<0.05).It was revealed by light microscopy that the retina was impaired in group B.The impairment of lens,ciliary body and retina in group B and D was detected by electron microscopy.Conclusion There may exist toxicity to the lens,ciliary body and retina in rabbit eyes after intravitreal injection of triamcinolone acetonide and vehicle.