四种标准菌株对两种氯己定类消毒剂的抗力研究

目的了解4种试验标准菌株对醋酸氯己定和葡萄糖酸氯己定的抗力。方法采用肉汤稀释法测定醋酸氯己定和葡萄糖酸氯己定对各试验菌的最小抑菌浓度和最小杀菌浓度。结果醋酸氯己定对金黄色葡萄球菌、大肠杆菌、白色念珠菌和铜绿假单胞菌的最小抑菌浓度分别为2、2、8和40 mg/L;最小杀菌浓度分别为512、1024、1024和1024 mg/L。葡萄糖酸氯己定对上述菌株的最小抑菌浓度分别为4、4、16和80 mg/L;最小杀菌浓度分别为1024、1024、2048和4096 mg/L。结论这两种消毒剂对4种试验标准菌株的最小杀菌浓度明显高于最小抑菌浓度。

STUDY OF RESISTANCE OF FOUR STANDARD BACTERIAL STRAINS TO TWO KINDS OF CHLORHEXIDINE DISINFECTANT

Objective To know the resistance of four standard bacterial strains to chlorhexidine acetate and chlorhexidine gluconate.Methods The broth dilution method was used to measure the minimum inhibitory concentration and minimum bactericidal concentration of chlorhexidine acetate and chlorhexidine gluconate to various testing bacterial strains.Results The minimum inhibitory concentrations of chlorhexidine acetate to Staphylococcus aureus,Escherichia coli,Candida albicans and Pseudomonas aeruginosa were respectively 2,2,8 and 40 mg/L.The minimum bactericidal(fungicidal) concentrations were respectively 512,1024,1024 and 1024 mg/L.The minimum inhibitory concentrations of chlorhexidine gluconate to these bacterial strains were respectively 4,4,16 and 80 mg/L.The minimum bactericidal(fungicidal) concentrations were respectively 1024,1024,2048 and 4096 mg/L.Conclusion The minimum bactericidal(fungicidal) concentrations of these two kinds of disinfectants to these four standard bacterial strains are obviously higher than the minimum inhibitory concentrations.