CMV-DNA detection in parenchymatous organs in cases of SIDS |
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| Authors: | Rosanna Cecchi Thomas Bajanowski Barbara Kahl Peter Wiegand |
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| Affiliation: | (1) Institute of Legal Medicine, Westfälische Wilhelms-Universität, Von-Esmarch-Strasse 86, D-48149 Münster, Germany;(2) Institute of Microbiology, Westfälische Wilhelms-Universität, Domagkstrasse 10, D-48149 Münster, Germany;(3) Institute of Legal Medicine, University of Rome, Viale Regina Elena 336, I-00161 Rome, Italy |
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| Abstract: | A nested PCR approach has been developed especially for the detection of small amounts of cytomegalovirus (CMV) DNA in autopsy samples. Lung tissue and submandibular glands in 118 cases of infant death (92 SIDS cases, 13 natural deaths due to other defined causes and 13 unnatural deaths) were investigated by this technique and compared to the results obtained by other CMV detection methods (histology, immunohistochemistry, in situ hybridization and PCR). CMV-DNA could be detected in the lung tissue in 7 cases of SIDS using nested PCR. Compared to conventional PCR (3 positive cases in lung tissue) the nested approach always gave glear results and showed less additional bands. In all cases where CMV could be detected in the lungs, positive results were also obtained in the submandibular glands. The nested PCR method proved to be a more sensitive technique than the other detection methods including PCR and hot start, and. even minimal amounts of target DNA could be detected in the presence of human and bacterial background DNA. |
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| Keywords: | CMV inclusion disease Lung tissue PCR Nested PCR |
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