Origin,migration and fate of newly generated neurons in the adult rodent piriform cortex |
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Authors: | Lee A Shapiro Kwan L Ng Richard Kinyamu Patricia Whitaker-Azmitia Eldon E Geisert Mathew Blurton-Jones Qun-Yong Zhou Charles E Ribak |
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Institution: | (1) Department of Anatomy and Neurobiology, School of Medicine, University of California at Irvine, Irvine, CA 92697-1275, USA;(2) Department of Pharmacology, School of Medicine, University of California at Irvine, Irvine, CA 92697-4625, USA;(3) Department of Psychology, Stony Brook University, Stony Brook, NY 11794-2500, USA;(4) Department of Ophthalmology, University of Tennessee Health Science Center, Hamilton Eye Institute, 930 Madison Avenue, Suite 710, Memphis, TN 38163, USA;(5) Department of Neurobiology and Behavior, Gillespie Neuroscience Research Facility, University of California at Irvine, Irvine, CA 92697-4540, USA |
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Abstract: | Newly generated neurons are continuously added to the olfactory epithelium and olfactory bulbs of adult mammals. Studies also
report newly generated neurons in the piriform cortex, the primary cortical projection site of the olfactory bulbs. The current
study used BrdU-injection paradigms, and in vivo and in vitro DiI tracing methods to address three fundamental issues of these
cells: their origin, migratory route and fate. The results show that 1 day after a BrdU-injection, BrdU/DCX double-labeled
cells appear deep to the ventricular subependyma, within the white matter. Such cells appear further ventral and caudal in
the ensuing days, first appearing in the rostral piriform cortex of mice at 2 days after the BrdU-injection, and at 4 days
in the rat. In the caudal piriform cortex, BrdU/DCX labeled cells first appear at 4 days after the injection in mice and 7 days
in rats. The time it takes for these cells to appear in the piriform cortex and the temporal distribution pattern suggest
that they migrate from outside this region. DiI tracing methods confirmed a migratory route to the piriform cortex from the
ventricular subependyma. The presence of BrdU/NeuN labeled cells as early as 7 days after a BrdU injection in mice and 10 days
in the rat and lasting as long as 41 days indicates that some of these cells have extended survival durations in the adult
piriform cortex.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Neurogenesis Olfactory system Doublecortin Bromodeoxyuridine DiI tracing |
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