变应性鼻炎大鼠鼻黏膜组织中血管细胞黏附因子-1与Eotaxin的表达及临床意义

摘要：目的分析血管细胞黏附因子 1(vasular cell adhesion molecule 1, VCAM 1)与Eotaxin在变应性鼻炎大鼠鼻黏膜组织中的表达及临床意义。方法SD健康大鼠40只随机分为2组:卵清蛋白(ovalbumin, OVA)致敏组和生理盐水(sodium chloride,SC) 组。以卵清蛋白致敏法建立变应性鼻炎大鼠模型,HE染色和甲苯胺蓝染色观察鼻黏膜组织病理学变化，免疫组织化学SP法测定鼻黏膜组织中VCAM 1、Eotaxin的表达。结果 OVA 致敏组可见鼻黏膜高度肿胀，大量炎性细胞浸润,SC组病理学未见异常表现；OVA 致敏组与SC组比较，VCAM 1和Eotaxin表达强度差异均具有统计学意义(P均<0.01); OVA 致敏组大鼠鼻黏膜组织中VCAM 1表达强度与Eotaxin呈正相关(r=0.886,P<0.01)。结论Eotaxin及VCAM 1参与了变应性鼻炎的发病过程，且二者有协同作用。

Expressions and clinical significances of vascular cell adhesion molecule 1 and Eotaxin in rat nasal mucosa of allergic rhinitis

Abstract:ObjectiveTo study the expressions and clinical significances of vasular cell adhesion molecule 1(VCAM 1) and Eotaxin in rat nasal mucosa of allergic rhinitis (AR).Methods40 Healthy SD rats were randomly divided into two groups: AR group and control group. Model of AR was established in rats with ovalbumin sensitization. HE staining and toluidine blue staining (TBS) were used to observe the pathological changes of the nasal mucosa. The expressions of VCAM 1 and Eotaxin were detected by SP immunohistochemical assay.ResultsPathological examination revealed obvious swelling of nasal mucosa with abundant inflammatory cell infiltration in AR group, and no abnormal changes in the control group. The expressions of VCAM 1 and Eotaxin in the AR group were significantly higher than those in the control group, and the differences were both statistically significant (both P<0.01). In the AR group, the expression of VCAM 1 was positively correlated with that of Eotaxin (r=0.886, P<0.01).ConclusionEotaxin and VCAM 1 may participate in the pathogenesis of AR in synergy.