丹酚酸B体外干预骨髓间充质干细胞分化过程中cTnT的表达

[目的]观察丹酚酸B(SalB)体外干预骨髓间充质干细胞(MSCs)后细胞形态及心肌特异性蛋白肌钙蛋白T(cTnT)的表达。[方法]培养、纯化及鉴定MSCs,用SalB、5-氮胞苷组(5-aza)、SalB联合5-aza(SalB 5-aza)分别定向诱导分化,观察此过程中MSCs的形态学变化并采用免疫细胞化学法鉴定诱导后MSCs心肌特异性肌钙蛋白T(cTnT)的表达。[结果]诱导后的MSCs体积增大,增殖减慢,并出现肌管样结构;免疫细胞化学结果显示诱导后MSCs表达心肌特异性蛋白cTnT。[结论]SalB在体外定向诱导MSCs分化为心肌细胞的过程中发挥了一定的作用。

Expression of cTnT induced by Salvianolic Acid B in differentiation of bone marrow Mesenchymal Stem Cells into myocardium-like cell

Objective To observe the effect of salvianolic acid B(SalB) in differentiation of bone marrow mesenchymal stem cells (MSCs) into myocardium-like cells. Methods MSCs was cultured,purified and evaluated,then MSCs was induced by 5-aza,SalB and 5-aza associating SalB to observe the cells' morphology change and evaluate the expression of cardiac troponin T(cTnT) by immunocytochemistry method. Results After induction,the volume of MSCs increased,the proliferation speed decreased. Myotube-like structure appeared and expressed cTnT. Conclusions SalB plays a certain role in the process of induction into myocardium-like cells.