类风湿关节炎四种自身抗体的检测及意义

为评估类风湿因子(rheumatoid factor,RF)、抗环瓜氨酸肽(cyclic citrullinated pepdide,CCP)抗体、抗Sa抗体和抗角蛋白抗体(anti-keratin antibody,AKA)自身抗体对类风湿关节炎(rheumatoid arthritis,RA)诊断的意义,采用速率散...

Significance of Determination of Four Autoantibodies for Diagnosis of Rheumatoid Arthritis

To investigate the diagnostic significance of four autoantibodies including rheumatoid factor(RF),anticyclic citrullinated peptide(CCP) antibody,anti-Sa antibody,and anti-keratin antibody in rheumatoid arthritis(RA),the serum sample of 123 patients with RA,105 patients with non-RA connective disease and 78 healthy subjects were involved in this study.RF and anti-CCP were quantitatively measured by velocity scattering turbidimetry and enzyme linked immunosorbent assay(ELISA) respectively.Anti-Sa antibody and AKA were detected by western-blotting and indirect immunofluorescence respectively.The results showed that the positive rate of RF,anti-CCP,anti-Sa and AKA for RA were 62.6%,69.9%,35.8% and 33.3%,respectively.The specificity for RF(79.8%) was significant lower than that of other three autoantibodies(94.5%,95.6% and 95.6%,P<0.01).The positive rates of anti-CCP,anti-Sa and AKA for RF negative patients were 41.3%,15.2% and 13.0% respectively.The positive coincident rate of RF and anti-CCP were 49.6% and kappa value was 0.501.The detection of more than two autoantibodies may greatly improve the specificity of RA diagnosis and could find the difference of expression of autoantibodies in different individuals during RA course.