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| 常见皮肤癣菌的基因分型 | |
| 引用本文： | 李金海,刘玉美,宫英,强新.常见皮肤癣菌的基因分型[J].青岛大学医学院学报,2007,43(3):269-270,272. |
| 作者姓名： | 李金海 刘玉美 宫英 强新 |
| 作者单位： | 1. 青岛大学医学院微生物学教研室,山东,青岛,266071 2. 青岛市市南区人民医院检验科 3. 潍坊医学院 |
| 摘 要： | 目的建立快速、简便鉴别4种常见皮肤癣菌（红色毛癣菌、须癣毛癣菌、絮状表皮癣菌和石膏样小孢子菌）的分子生物学基因分型方法。方法将我室分离培养得到的18株红色毛癣菌、12株须癣毛癣菌、8株絮状表皮癣菌和6株石膏样小孢子菌接种于PDA培养基上培养2周后,分别收集菌丝体并用氯化苄法提取DNA,运用随机扩增多态性DNA分析的方法,以单个引物（GACA）4进行随机扩增。检测聚合酶链反应产物,并根据电泳后指纹图谱的差异进行种属间鉴别。结果同种不同株的皮肤癣菌电泳后指纹图谱相同,不同种皮肤癣菌电泳后指纹图谱有显著差异。结论运用随机扩增多态性DNA分析的方法,可对红色毛癣菌、须癣毛癣菌、絮状表皮癣菌和石膏样小孢子菌的基因型进行快速鉴别,能够协助临床早期诊断。 |
| 关 键 词： | 表皮癣菌属 基因型 随机扩增多态DNA技术 实验室技术和方法 |
| 文章编号： | 1672-4488（2007）03-0269-03 |
| 修稿时间： | 2006-11-152007-01-15 |
ESTABLISHMENT OF THE GENOTYPING METHOD FOR COMMON DERMATOPHYTES |
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| LI JIN-HAI, LIU YUMEI, GONG YING, el. al.ESTABLISHMENT OF THE GENOTYPING METHOD FOR COMMON DERMATOPHYTES[J].Acta Academiae Medicinae Qingdao Universitatis,2007,43(3):269-270,272. | |
| Authors: | LI JIN-HAI LIU YUMEI GONG YING el al |
| Institution: | Departmem of Microbiology, Qingdao University Medical College, Qingdao 266071, China |
| Abstract: | ObjectiveTo establish a rapid,reliable method to distinguish common species of dermatophytes.Methods Random amplification of polymorphic DNA(RAPD) and a simple repetitive oligonucleotide(GACA)4 were used to amplify the DNA of Trichophyton rubrum(n=18),Trichophyton mentagrophyte(n=12),Epidermophyton floccosum(n=8) and Microsporum gypseum(n=6)ResultsThe DNA of all the strains of dermatophytes was amplified successfully,producing species specific profiles.Conclusion The random amplification of polymorphic DNA for identification of common species of dermatophytes is a stable,prompt and sensitive method.ConclusionEpidermophyton;genotype;random amplified polymorphic DNA technique;laboratory technics and procedures. |
| Keywords: | epidermophyton genotype random amplified polymorphic DNA technique laboratory technics and procedures |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |