EB病毒对人胃癌细胞系HSC-39感染的研究

目的 探讨EB病毒（EBV）对胃癌细胞系的感染作用。方法 用Akata和P3HR－1 EBV毒株感染人胃癌印戒细胞系（HSC－39），有限稀释法对感染细胞进行克隆。结果 EBV感染细胞中可检测到EBV编码的核抗原（EBNA)，2种EBV毒株感染的细胞克隆表现有不同的形态学特征及生长方式。EBV感染的亲代细胞及大部分克隆表达EBNA1，但不表达EBNA2、潜伏期膜蛋白（LMP）1和LMP2A；亲代细胞及所有细胞克隆未观察到裂解感染，EBNA启动子Qp表达阳性，而启动子Cp和Wp未见表达。结论 HSC－39对2种EBV毒株均易感，EBV感染可改变HSC－39的细胞表型，且不同EBV毒株对其影响不同，提示印戒细胞癌细胞系可用作EBV感染的靶细胞。

Epstein-Barr virus infection of a human signet ring cell gastric carcinoma cell line

Objective To study Epstein-Barr virus (EBV) infection on gastric carcinoma cells. Method A signet ring cell line HSC-39 derived from human gastric carcinoma was infected with Akata and P3HR-1 EBV strains. Akata and P3HR-1 EBV infected cell clones were isolated by a limiting dilution method. Results EBV-encoded nuclear antigen (EBNA) was detected in the infected cells. The Akata and P3HR-1 infected clones showed heterogenous morphology and growth pattern. The EBV infected parental cells and most clones expressed EBNA1, but not EBNA2, latent membrane protein (LMP) 1 and LMP2A. The Q promoter (p) but not Cp/Wp was active in the infected parental cells and all clones. No lytic infection was observed in the infected parental cells and all clones. Conclusion HSC-39 cells were susceptible to the both EBV strains. The phenotypes of HSC-39 cells were altered by EBV infection and the alteration depends on the EBV strains. This study defined a signet ring cell line as a new target for EBV with a unique infection.