| 组织块法培养人绒毛膜滋养层细胞 |
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| 引用本文： | 王雪萍,徐德忠,李远贵,闫永平,罗深秋.组织块法培养人绒毛膜滋养层细胞[J].南方医科大学学报,2001,21(1):57-59. |
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| 作者姓名： | 王雪萍 徐德忠 李远贵 闫永平 罗深秋 |
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| 作者单位： | 1. 第四军医大学流行病学教研室,
2. 第一军医大学细胞生物学教研室, |
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| 基金项目： | 国家自然科学基金!（398001） |
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| 摘 要： | 目的培养符合实验要求的人绒毛膜滋养层细胞。方法用组织块法行原代培养，胰蛋白酶消化法行传代培养，免 疫组织化学染色进行细胞鉴定。结果６ｄ时，细胞开始由组织块边缘向外生长；１５ｄ时，即可进行传代。角蛋白染色示细 胞纯度达９５％以上。结论组织块法培养人绒毛膜滋养层细胞简便易行，可获得符合实验要求的人绒毛膜滋养层细胞。
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| 关 键 词： | 滋养层细胞 绒毛膜 细胞培养 |
| 文章编号： | 1000-2588(2001) 01-0057-03 |
| 修稿时间： | 2000年6月12日 |
In vitro culture of human trophoblast cells by tissue-cultutre |
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| WANG Xue-ping,XU De-zhong,LI Yuan-gui,YAN Yong-ping,LUO Shen-qiu.In vitro culture of human trophoblast cells by tissue-cultutre[J].Journal of Southern Medical University,2001,21(1):57-59. |
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| Authors: | WANG Xue-ping XU De-zhong LI Yuan-gui YAN Yong-ping LUO Shen-qiu |
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| Abstract: | Objective To obtain human trophoblast cells suitable for future experiments by in vitro cell culture. Methods Primary culture of human trophoblast cells was carried out by tissue-culture method,and the subsequent serial passage of the cells performed by trypsin digestion, identified and assayed by immunohistochemical staining. Results Growth of the cells from the minced tissue was observed at around day 6, and cell passage could be performed at day 15 approximately. Keratin staining showed that the purity of the cultured cells exceeded 95%. Conclusion Tissue-culture method is techmically less demanding while the cells obtained thereby may well satisfy subsequent experiments. |
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| Keywords: | trophoblast cells chorion: tissue-cultrue |
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