蝙蝠葛酚性碱对心肌缺血及缺血再灌注损伤的保护作用

目的研究蝙蝠葛酚性碱(PAMD)对心肌缺血及缺血再灌注损伤的作用。方法用大鼠皮下注射异丙肾上腺素85 mg.kg-1造成心肌缺血模型,于造模前30 min灌胃给予生理盐水、PAMD(7.5,15或30 mg.kg-1)、地尔硫10 mg.kg-1或地奥心血康150 mg.kg-1,测定心肌缺血后乳酸脱氢酶(LDH)、肌酸激酶(CK)、超氧化物歧化酶(SOD)和丙二醛(MDA)含量及病理学改变。离体大鼠心脏缺血30 min再灌注40 min,分别给予PAMD(1,10或100 mg.L-1)或地奥心血康100 mg.kg-1,测定心肌缺血再灌注后左室收缩压(LVSP)、左室舒张末压(LVEDP)和左室压最大变化速率(±dp/dtmax)、冠脉流量,测定心肌SOD活性和MDA含量。结果 PAMD显著降低缺血所致的LDH、CK和MDA升高,提高SOD活性,减轻心肌病理损伤。PAMD促进缺血再灌注末LVSP、LVEDP和±dp/dtmax等恢复,增加冠脉流量和SOD活性并降低MDA含量。结论 PAMD对心肌缺血及缺血再灌注损伤有保护作用,其机制可能与其抗氧化及改善心肌代谢有关。

Protective effects of phenolic alkaloids of Menispermum Dauricum on ischemia and ischemia - reperfusion injury

Objective To investigate the effects of phenolic alkaloids of Menispermum Dauricum(PAMD) on myocardial ischemia and ischemia-repurfusion in rats.Methods Myocardial ischemia in SD rats were induced by isoprenaline injection(isoprenaline,85 mg·kg-1).Saline,PAMD(7.5,15 or 30 mg·kg-1),diltiazem 10 mg·kg-1or Diaoxinxuekang(DAXXK) 150 mg·kg-1 were administrated intragastriclly at 30 min before isoprenaline injection.The level of lactate dehydrogenase(LDH),creatine kinase(CK),super oxide dismutase(SOD),and malondialdehyde(MDA) were measured and myocardial pathological grade were evaluated.The langendorff perfused rat hearts were subjected to 30 min of global ischemia following by 40 min reperfusion.PAMD(1,10 or 100 mg·L-1)or DAXXK 100 mg·L-1 were added into the modified KH solution.The changes of left ventricular systolic pressure(LVSP),left ventricular end dilated pressure(LVEDP),maximum rising and failing rate of left ventricular pressure(±dp/dtmax),the flow of coronary artery,SOD and MDA were measured.Results In myocardial ischemia rats,PAMD obviously decreased the level of LDH,CK and MDA in serum,increased the activity of SOD and improved the pathological grade of myocardial lesion.After subjected to ischemia-reperfusion,PAMD improved the recovery of the index of contraction and stretch,such as LVSP,LVEDP and ±dp/dtmax.It also increased the flow of coronary artery and the activity of SOD,as well as decreased the content of MDA in myocardium.Conclusion PAMD can attenuate ischemia and ischemia-reperfusion injury in rat hearts.